Further Experiments, &c. By Dr. R. L. Maddox. 945 



amination ; they furnished a large amount of scaly granular matter, 

 some extremely fine motionless rods and a few well-marked curved 

 bacilli, some in the double or S-shape, but without distinct move- 

 ment, and the acicular crystals were very rare. 



Early in the morning of the 10 th, the flies were found in coitus, 

 and at 1.30 p.m. the female was found dead. Before the fly 

 was removed, the male made repeated attempts at coitus with 

 his dead mate. It was not until five hours later that I could 

 attend to the examination of the fly. Upon making a section of 

 the posterior end of the abdomen and placing it in diluted potassic 

 acetate solution, numerous spermatozoa, in bundles and free, were 

 noticed. In the perivisceral fluid there was much fine granular 

 matter, a very few short and stout, and also some thin motionless 

 rods, and scarcely a curved bacillus to be found. When the contents 

 of the cavity were removed with a mass of the ova capsules, the fine 

 granular matter, possibly from their rupture, even when much 

 diluted, was so abundant that nothing satisfactory could be deter- 

 mined. The death of this fly appeared to be due to the feeding for 

 six days upon the anilin stained sugar, which it ill supported com- 

 pared with Eristalis. The male fly was now detained by itself, 

 and fed from the original agar-agar culture diluted on the sugar. 

 The daily dejections when examined showed some short, straight, 

 thick non-motile rods, some bright oval spores, a few germinating, 

 and abundant micrococci. The comma bacilli present were few, 

 and none seen to be motile ; no acicular crystals. On the 15 th, 

 nine dejections found on the square were mingled, they were in 

 whitish patches and quite dry ; the micrococci were very numerous, 

 with scarcely a straight rod present, and a few motionless commas, 

 some double with the curvature on the same side. Five dejections 

 of the 16th furnished a rather large number of the curved bacillus, 

 more than in any previous dejections, and some of them had a rather 

 sluggi.ih motion, many appeared to be somewhat short and dumpy 

 in shape or immature, a few were double ; the straight rods were 

 fewer, but the micrococci were numerous. It was found exceedingly 

 difficult to so apportion the moisture that the sugar should remain 

 just damp for some hours. The fly would often turn the small 

 lump over, seeking for the moistest spot. 



On the 17th eighteen dejections had been passed by the fly in 

 less than twenty-four hours, they were dry, but when mingled 

 together in a droplet of fre.shly boiled distilled water furnished 

 several little colonies or clusters, containing from five to seven 

 curved bacilli, also single and double ones. They varied a little in 

 size and curvature ; scarcely any of the short, thick, and straight 

 rods were visible, and only a few fine small straight rods. As the 

 fly had now been under observation for some days, and seemed 

 lively, it was killed by chloroform vapour, in order to examine the 



