952 Transactions of the Society. 



from a fine pipette. Great care was taken for cleanliness of all the 

 vessels. The gelatin preparations, generally used for the rejuve- 

 nescence of the microbes, contained 10 per cent. Liebig's extract of 

 meat neutralized by soda and potash or soda alone, with 10 per 

 cent, hard gelatin, and no peptone. The fluid cultures had 12 per 

 cent, of meat extract. These probably were not the best formulae 

 for the object in view, but I had rather to hinder than encourage 

 growth, on account of the micrococci. 



The beetle alluded to in the former paper died a few days 

 afterwards; the excretions were of the same character as those 

 described. The body was not examined. It will be readily seen 

 that such investigations demand considerable time, and the dijfi- 

 culties to convert a simple supposition into a demonstration have 

 been both tiresome and numerous. 



