ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 897 



Ganglion-cells become bright blue ; the nuclei and nucleoli dark blue, 

 the processes as well as the cell-body being stained. 



3. Anilin-blue-black is of no value for connective tissue and the 

 neuroglia ; for these the author uses alum-cochineal, hematoxylin, or 

 the Bottcher-Hermann anilin dye method. 



4. The staining solutions are very simple. The author uses three 

 watery solutions, 1 in 100, 1 in 800, and 1 in 2000, which stain in 

 four, five, and twelve hours respectively. Then alcohol, oil, and 

 balsam. 



5. Anilin-blue-black tires the eyes much less than carmine, an 

 advantage not to be undervalued when a large number of serial 

 sections are to be compared. 



Anilin-green. — Dr. P. Schiefferdecker's first communication * on 

 this subject was to the effect that solutions of anilin-green undergo a 

 certain change of composition from exposure to light, in virtue of 

 which alteration they acquire a peculiar susceptibility for staining 

 gland-tissue. This peculiar change cannot be effected in any other 

 way than by age and exposure to light ; the addition of' alkalies or 

 acids, the aid of gentle heat and various degrees of concentration, 

 make no difference in the capacity of a fresh solution. 



Since the first communication, the author has made experiments,! 

 in order to obtain a record of the time the blackish-green reaction 

 takes to develope. In twelve months a solution of anilin-green gave 

 results which were about half-way between those of the seven-year old 

 solution, and of the solution freshly made. Iodine-green, malachite- 

 green, emerald-green, and several methyl-greens, were used in the 

 course of the author's investigations on the salivary glands. But 

 one methyl-green, prepared by the Stuttgart Anilin-Soda-Fabrik, and 

 designated OO, produced in fresh solution results very similar to 

 those from the old solutions. The author thinks the blackish-green 

 reaction of anilin-green to be quite specific and of great value. 



Anilin-greens may be mixed with eosin, so as to stain a preparation 

 red and green simultaneously. The double stain is made by allowing 

 some alcoholic eosin solution to dry up in a watchglass and then to 

 add the anilin-green solution. By this method so much eosin is taken 

 up as is necessary to combine with the anilin-green for the production 

 of a double stain. Methyl-green O O gives similar good results. 



Modification of the Formula for Alum-Carmine. J — Dr. Pisenti 

 recommends the following modification of the formula for the alum 

 carmine first introduced by Grenadier. In 100 c.cm. of a hot satu- 

 rated watery solution of alum (100 parts boiling water dissolve 133 

 parts crystallized alum) 1 • 5 to 2 grm. carmine are allowed to boil for 

 a few minutes ; 2 grm. of sulphate of soda are then added. This dis- 

 solves the small residue of carmine which the alum solution has left 

 undissolved. It is then boiled again for five minutes and filtered 

 while hot. The fluid is then allowed to cool, and as a considerable 



* Zeitschr. f. Wiss. Mikr., ii. (1885) pp. 51-3. f Ibid., iii. (188G) pp. 41-3. 



X Gazzetta degli Ospitali, 1885, No. 24. Cf. Zeitschr. f. Wiss. Mikr., ii. 

 (1885) p. 376. 



Ser. 2,-Vol. VI. 3 N 



