ZOOLO&Y AND BOTANY, MIOEOSCOPY, ETC. 



657 



Fig. 108. 



pure 8 per cent, casein solution, quite free from fat, are mixed with 

 350 ccm. of a filtered mixture of whey and 12 per cent, gelatin or 

 1-75 per cent. agar. The whole mass is then heated to 60° C. and 

 transferred to test-tubes. 



To prepare milk-albumen-gelatin and milk-albumen-agar the peptone 

 is replaced by a saturated solution of sodium albuminate. 



With the foregoing media cultivation experiments were made with 

 Bacillus mallei, B. Typli. ahclom., comma bacillns, B. tussis convids. 



The authoress states that glanders-bacillus developes luxuriantly on 

 the milk-peptone media at 37°-38° C. On the second day after inocula- 

 tion a thick dull-white crust forms on the agar surface. In three to four 

 days the colour is amber to orange, the deeper layers being brownish - 

 red. The authoress is disposed to regard these milk media as being 

 very favourable to the growth of certain microbes which on others do 

 not betray any special characteristics. 



Vessel for the Culture of Low Organisms.* — Herr N. W. Diakonow 

 has constructed an apparatus, of which the following is a description, 

 for the culture of low organisms, the special object being to prevent the 

 intrusion of bacteria and other foreign bodies. 



The apparatus (fig. 108) consists of a vessel composed of two parts, 

 a bulb A provided with two necks, and a burette B, connected with one 

 another by a caoutchouc tube in such a 

 way that the burette moves easily from side 

 to side. To the lower end of the burette, 

 which must be supplied with a glass tube 

 of equal diameter with the upper portion 

 of the neck of the bulb, is fused a short 

 and narrow glass tube running out into a 

 capillary prolongation. The upper part 

 of the burette is again connected with a 

 narrow glass tube by means of a caou- 

 tchouc tube shut off by a stop-cock, the 

 glass tube being widened at its upper end 

 for the reception of a wad. The size of the 

 entire apparatus may be adapted to the 

 requirements of the experiments ; for fungi 

 cultivated on a nutrient solution only 

 10-15 ccm. in quantity, the height need 

 not exceed 15-17 cm. ; the bulb then 

 having a cajjacity of about 70-80 and the 

 burette of about 3-5 ccm. It is especially 

 needful that the apparatxis should be so 

 constructed that, after the sterilizing of the 

 nutrient solution, no foreign organisms can 

 enter it. 



In using the apparatus, the burette and 

 the solution to be introduced into it must 

 first of all be sterilized. For this purpose 

 the whole burette with its capillary pro- 

 longation is dipped into boiling water, which is sucked up to the upper 

 bulb containing the wad ; this process is repeated several times. The 



* Ber. Deutsch. Bot. Gescll., vi. (1888) pp. 52-1 (1 lig.). 



