ZOOLOGY AND BOTANY, MICKOSCOPYj ETC. 831 



Klemensiewicz, K. — Ein Vegetationskasten fiir niedrige Temperatnr. (A 

 culture chamber for low temperatures.) Wiener Klin. Wochenschr., 1888, p. 283. 



NoEGGERATH, E. — TJeber eine neue Methode der Bacterienziiclitung auf gefarbten 

 Nahrmedien zn diagnostisclien Zwecken. (On a new method of bacteria culti- 

 vation on coloured nutrient media for diagnostic purposes.) 



Fortschr. d. Med., VI. (1888) pp. 1-3 (1 pi.). 



Unna, p. G. — Die Zuchtung der Oberhautpilze. (The cultivation of skin fungi.) 



Monatschrift fur Prakt. Dermatol., 1888, pp. 465-76. 



Zagaei, G. — La Coltura del Micro-organisme AnaeroM. (The culture of anaerobic 

 micro-organisms.) Giorn. Internaz. Sci. Med., 1888, p. 218. 



(2) Preparing Objects. 



Eifect of Hardening Agents on the Ganglion-cells of the Spinal 

 Cord.* — Dr. S. Trzebinski Las experimented on a number of hardening 

 media to ascertain whether and in what way they affect the ganglion- 

 cells of the spinal cord in rabbits and dogs. 



(1) Miiller's fluid : hardening 4 to 5 weeks. The preparations were 

 either washed before being placed in spirit, or were placed in spirit in 

 the dark without being washed. The spirit was from the first of 96° or 

 it was made weak (10°), and increased in 5 days to 96°. 



(2) Hardening in spirit either of 96° at once or by increased 

 strengths as in No. 1. 



(3) Hardening in chromic acid. The preparations were placed for 

 6 hours in a 0*1 per cent, solution, then for 48 hours in a 0*25 per 

 cent, solution, and were afterwards hardened in spirit or in a mixture of 

 Miiller's fluid and spirit. 



(4) Hardening in 10 per cent, sublimate solution (8 days) with 

 subsequent hardening in spirit which contained • 5 per cent, iodine. 



The stains used were, borax-carmine, alum-carmine, with or without 

 previous staining in Weigert's haematoxylin solution, magenta-red, and 

 Weigert's method. Fresh preparations were coloured with methyl-green. 

 In fresh preparations stained with methyl-green the ganglion-cells were 

 on the whole well stained, their finer structure recognizable, and there 

 was no evidence of pericellular spaces. In all the preparations treated 

 by the above hardening methods the ganglion-cells were altered, (1) peri- 

 cellular spaces appeared ; (2) vacuoles in the cell-substance ; (3) the cell 

 contents did not show the same structure as in the fresh cells ; (4) the 

 susceptibility of the cell contents for dyes had become inconstant. On 

 the whole the most satisfactory method seemed to be the sublimate 

 process which was followed by iodized alcohol. 



Sublimate as a Hardening Medium for the Brain.f — Herr A. 

 Diomidoff hardens brains and cords in 7 per cent, watery sublimate 

 solution. The preparations, which should not be larger than 1 ccm., are 

 left in the solution not longer than five to nine days, and then passed 

 through successively 50°, 70°, and 90° spirit. In each spirit the pre- 

 paration remains about twenty-four hours, so that the whole hardening 

 occupies about eight days. 



The chief point in the author's paper consists in his observation 

 that all hardening fluids which contain mercury salts alone or in com- 

 bination with silver solutions, or solutions of the latter in combination 

 with chromic or copper salts, produce after long action on nerve prepara- 



* Vtrchow's Archiv, cvii. (1887) pp. 1-17. 



t Wratsch, 1887, pp. 472-4. Of. Zeitschr. f. Wiss. Mikr., iv. (1887) pp. 499-500. 



