834 SUMMARY OF CURRENT RESEARCHES RELATING TO 



Ben DA, C. — Eine neue Hartungsmethode besonders fur das Centralnervensystem. 

 (A new hardeuing method especially for tlio central ncrvoua system.) 



Ccntr.dbl. Mr J. ll'i^s., XXVI. (1S88) p. 497. 

 G IE SON, J. VAN. — A Eesume of recent Technical Methods for the Nervous System. 

 Journ. Ncrv. and Mental Diseases, XIV. (1887) p. 310. 

 G I F F o R n. J. W. — Preparations for High Powers. 



[Btale's glycorin-oarmine fluid — Gum and glycerin and glycerin jelly — Modifi- 

 catiou of Flcmming's chromo-aceto-osraic acid.] 



Jow-n. of Micr., I. (1888) pp. 152-4. 

 Klein, L. — Beitrage zur Technik der mikroskopischen Danerpraparate. (Contri- 

 butions to the technique of permanent microscopical preparation.-*.) 



MT. Hot. Vcreins Frelbur;, 188S, Nos. 49-50. 



RvDANOMSKT. — Making Microscopical Nerve Preparations by dividing the nerves 



into primitive bundles by chemical processes, and the latter into their component 



parts. Jiussl-aja Mcdichvi, 1887, Xo. 38 (Ruisiau) 



WooDUEAD, G. S. — Method of preparation of large sections of the Lung. 



Brit. Med. Journ., 1888, p. 737. 



(3) Cutting, including: Imbedding. 



Photoxylin for Imbedding. — Dr. Krysinski * suggests the use as an 

 imbedding substance of jihotoxylin, a kind of pyroxylin used by Russian 

 photographers, and which he considers superior to celloidin on account 

 of its keeping without deterioration, and remaining clear in solution or 

 mass. Mr. G. M. Beriuger,"]" who has experimented in the production of 

 photoxylin, finds that the following formula gives the best results : — 

 Nitrous acid, 43° E., 3^ lb. av. ; sulphuric acid, 4^ lb.; potassium 

 nitrate, granular, 8 oz. ; wood pulp, 4 oz. 



The nitrous and sulphuric acids are mixed in an earthenware crock 

 and allowed to stand until the temperature has fallen to 90^ F., when 

 the potassium nitrate is added and thoroughly incorporated with the acid 

 mixture. The wood pulp is then immediately immersed in the mixture 

 and allowed to remain for twelve Lours. It is then removed from the 

 acid and thoroughly washed. 



The material thus obtained is quite soluble in equal parts of ether 

 and absolute alcohol. For general work Krysinski recommends two 

 solutions; a thin solution (1/2 to 1 per cent.), and a 5 per cent. The 

 specimen is placed from strong alcohol into the thin solution, to remain 

 from twelve to twenty-four hours, when it is transferred to the thicker 

 solution. To fix the siiccimen before cutting, it is only necessary to 

 place it on a cork. A film soon spreads over the mass, which is then 

 submerged in 70 per cent, alcohol, and after two or three hours is ready 

 for sectioning. 



Paraflin-imbedding Process in Botany^ — "Within a few months 

 tbere have appeared two articles § on this subject, and as Mr. D. H. 

 Campbell has been devoting some attention to it lately, he thinks it may 

 •be of interest to state briefly tbe results obtained. It was found con- 

 venient to combine to some extent the methods given in the articles 

 referred to, as neither was found in all respects satisfactory, and some 

 simplifications of the processes were made which were foimd advan- 

 tageous. 



* Vircbow's Arch. f. Path. Anat. u. Hist., 1888. Cf. The Microscope, viii. (1888) 

 p. 183. t Amer. Journ. Pharm., 1888. Cf. ibid. 



X Eot. Gazette, xiii. (1888) pp. 158-60. 



§ Schonland, S., l^t. Centralbl., xxx. (1887) pp. 283-5. See this Journal, 1887, 

 p. 680. Moll, Dot. Gazette, xiii. (1888) pp. 5-14. Sec this Journal, ante, p. 315. 



