842 SUMMARY OF CURRENT RESEARCHES RELATIKG TO 



pressure. The under surface of the paper baud is rendered adhesive by 

 nieaus of gum aud collodion, and thus by each action of the knife a new 

 section is placed in position along the band, the front end of which must 

 be snipped off to remove the piece carrying the section, and then 

 reclamped. 



Baltzar, G., and E. Z i m m e r m a n x.— Microtom mit festem Messsrund selbst- 

 thatigem Vorschub des Objekts. (Microtome with fixed knife and automatic 

 niovenieut of the object.) German Patent, Kl. 42, No. 1431, 1888. 



rMANTON, W. P., and others.]— Modem Methods of Imbedding. 



ne Microscpe, VIII. (1888) pp. 181-4. 



6 TO WELL, C. H.— Thin Sections. The Microscope, VIII. (1888) pp. 175. 



C4) Staininer and Injectiner. 



Double-staining of Nucleated Blood-corpuscles.*— Dr. W. M. Gray 

 gives tlie following directions : — S2)read a thin layer of blood on a 

 clean slide aud dry. Immerse the slide in a beaktr of alum-carmine 

 (Grcnachcr's formula) for five minutes ; wash in clean w^ater, and 

 immerse in a beaker of a weak solution of siilph-indigotate of soda or 

 potash (the solution should be of a dark-blue colour — not black-blue, as 

 in a strong solution). After the slide has acquired a purplish hue, wash 

 in water and dry. After drying, warm slightly and mount in balsam. 

 The nuclei will be a beautiful red, and the protoj)lasm a gi'ecnish 

 blue. 



Vital Methylen-blue Reaction of Cell-granules.j — If the larva) of 

 the frog or triton, says Dr. 0. Schultze, be placed in a watery solution 

 of pure methylen-blue, of the strength of 1 : 100,000-1,000,000, after 

 twenty-four hours, certain granules in the cells of the cutaneous epithelium 

 become stained with the weakest solution ; the staining is confined to 

 a small spot close to the pylorus which to the naked eye resembles a 

 small blue ring. When the strongest solution is used for eight days, 

 all the parts become of a deep blue colour. The pigment is absorbed by 

 certain granules within the cells and causes them to swell up. These 

 are identical with Altmann's bioblasts. These granules are not stained, 

 or at any rate very slightly, when the dye is introduced through the 

 blood-current, while, on the other baud, in larvae living in the blue 

 sjlution, the nerves are not stained. If the larvae be removed from the 

 blue solution to pure water all trace of the pigment disappears in 

 eight days. 



DiJfferential Staining of the Tissues of Living Animals.J — M. A. 

 Pilliet has found that, by a simple subcutaneous or iutra-peritoneal 

 injection of methyl-blue, in rats, guinea-pigs, and other small animals, 

 the entire kidney and some other organs are stained a diffused blue. 

 By mixing the same material (methyl-blue) with the food of rats and 

 guinea-pigs, only the glomerules of the kidneys were stained. If, 

 instead of blue, fuchsin be used, the entire kidney becomes stained a 

 vinous red, which, under section, however, shows the glomerules and 



♦ Queen's Micr. Bulletin, v. (1888) p, 15. 

 t Anat. Auzeig., ii. (1887) pp. 684-8. 



X tSt. Louia Med. and Surg. Journ., Iv. (1888) pp. 28-9 from ' Progres Me'dical.' 

 Cf. also Journ. de Microgr., xii. (1888) pp. 285-90. 



