1056 SUMMARY OF CURRENT RESEARCHES RELATINQ TO 



tion and its bearing on karyokinesis, the author found that it did 

 not seem to have any direct relation to the nuclear mitosis. In order 

 to fix the mcthylcn-blue in the tissues an iodized solution of iodide 

 of potash, or picrocarmine, or picrocarminate of ammonia, was used, 

 the preparations being afterwards mounted in glycerin. This method 

 was found to bo inconvenient. 



If Bismarck -brown be used, the tadpoles were immersed alive in a 

 0*2 per cent, solution of chromic acid. This fixed the tissues without 

 affecting the Bismarck-brown. The tissues were then washed, and 

 afterwards stained with safranin. In using spirit it is necessary to 

 be cautious, as it rapidly absorbs the dye. 



Theory of Microscopical Staining.*— Dr. H. Griesbach says that tho 

 more lie considers the subject of microscopical staining the more he is 

 convinced that it is based on chemical combinations taking place between 

 the tissues and the pigments, both of which must, for various reasons, 

 have very different chemical compositions at different times. This is 

 easily obvious from certain examples, say, the composition of the infantile 

 and adult brain. This difference in chemical composition is further 

 augmented by the various reagents used for fixing the tissues, and also 

 complicated by the reaction and composition of the dye itself. And so on. 



Starch Injection-mass.f — Prof. S. H. Gage prepares a cold-flowing 

 coarse injection-mass, the principle of which was first introduced by 

 Ad. Pansch, from starch. This mass may be forced up nearly to tho 

 capillaries, rapidly hardens after injection, leaves the vessels flexible, 

 and is suitable for permanent dry or alcoholic preparations. 



Mass for ordinary injection : dry (laundry) starch, 100 ccm. ; water or 

 2J per cent, aqueous solution of chloral hydrate, 100 ccm ; 95 per cent, 

 alcohol, 25 ccm. ; colour mixture, 25 ccm. When thoroughly mixed filter 

 through two or three thicknesses of cambric. To prevent the starch 

 from settling, the cloth should be tilted from side to side or the mass 

 stirred during filtration. 



The colour mixture : dry colour (e.g. Berlin blue), 100 ccm. ; glycerin, 

 100 ccm. ; 95 per cent, alcohol, 100 ccm. Mix well in a mortar and keep 

 in stoppered bottle. If permanent preparations are not desired, anilin 

 dyes may be used. 



Special injection-mass for brains, &c. : corn starch, 100 ccm.; 5 per 

 cent, aqueous solution of chloral hydrate, 50 ccm. ; 95 per cent, alcohol, 

 75 ccm. ; colour mixture, 25 ccm. Either of the masses may be kept in 

 large quantities in wide-mouthed bottles, but must be well stirred before 

 using. If it be desired to inject very fine vessels, a preliminary injection 

 should be made by using the stock mass diluted with an equal volume 

 of water or of chloral solution. In any case it is advisable to make the 

 injection as quickly as is possible. 



Ac HARD, C. — Sur Temploi de la teintore d'orcanette dans la techniqae Mstologique. 

 (On the employment of a tincture of orcanet in histological technique.) 



Arch, de Physiol., IX. (1887) pp. 16i-8. 

 Erdos, J. — Eine Methode znr Injection der Blutgefasse mit kaltfliissiger Masse. 

 (A method for the injection of the blood-vessels with a cold fluid mass.) 



Anat. An-m.j., III. (1888) p. 261. 



Kertesz, a. — Die AnilinfarhstofPe. Eigenschaften, Anwendung, Eeactionen. 



(The Anilin stains. Properties, use, reactions.) 8vo, Braunschweig, 1888. 



* Zeitschr. f. Wiss. Mikr., v. (1888) pp. 314-19. 

 t Amer. Mon. Micr. Joum., ix. (1888) pp. 195-6. 



