ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 311 



are merely washed clean. Cubes of apple or potato, sufficiently large 

 so as not to interfere with one another in the tubes, are then cut up. 

 About eight of these cubes are able to be put in each tube, and the latter 

 having been plugged with cotton wool, are placed in a steam sterilizer 

 for half an hour. When cool, transfer to a well-closed jar, upon the 

 bottom of which some water must be poured from time to time. Here 

 they may be kept for quite a month. Thus, after sterilization, is 

 obtained from four tubes material sufficient for 32 Koch's jars. Each 

 cube is removed to a separate test-tube or jar by impaling it on the bent 

 end of a piece of platinum wire previously thoroughly heated. Some 

 practice is needful for this, as the cubes are apt to slip away. The 

 cover of the jar may be held up by an assistant, or more simply the 

 whole manipulation may be affected as described by the author in 

 Ziirn's ' Parasiten,' 2nd edition, ii. p. 165. The apple-cubes, which the 

 author uses for cultivating all kinds of Saccharomyces, become soft as 

 jelly after sterilization, and are only held together by the peel. Hence 

 manipulation of them is somewhat troublesome, but if any irregularity 

 of surface occur, this may be removed by smoothing it down with a 

 previously heated spatula. 



To obtain and keep a quantity of water that shall be free frcm fungi, 

 the author takes an ordinary flask ; this is three-parts filled with water, 

 plugged with cotton wool, and sterilized. The rubber tube and the glass 

 stoppers are then fitted in and plugged round with cotton wool. The 

 apparatus is then placed in a steam sterilizer for half an hour. When 

 cool, the one end is fitted with a rubber spray bellows, and the other 

 supplied with a pinchcock. When to be used it is necessary to squeeze 

 the bellows twice before opening the pinchcock, and to close the latter 

 before the stream of water has ceased. 



Arloing — Modification apportee a un analyseur bacteriologique. (Modification 

 in a bacteriological analyser.) CR. Soc. Biol., 1SS7, p. 722. 



Dal Pozzo, D. — Das Eiweiss der Kiebitzeier als Nahrboden fur Mikroorganismen. 

 (The albumen of the plover's egg as a culture medium for micro-orgauisms.) 



Med. Jahrb. ( Wien), 1887, pp. 523-9. 



Fischl, R. — (a) Ein neues Verfanren zur Herstellung mikroskopischer Praparate 

 aus Reagenzglasculturen ; ('>) Die Anfertigung von wirksamen mit Mikro- 

 organismen impragnirten Faden. ( (a) A new process for making microscopic 

 preparations from test-tube cultures ; (6) the preparation of threads effectively 

 impregnated with micro-organisms.) Fortschr. d. Medicin, 1887, pp. 663-6. 



Roux, E. — De la Culture sur Pomme de terre. (On potato cultivation.) 



Ann. Instit. Pasteur, 1888, pp. 28-30. 



(2) Preparing Objects. 



Demonstrating the Reticulated Protoplasm in the Interstitial 

 Cells of the Ovary.* — M. N. Lowenthal remarks that it is not rare to 

 meet in sections of ovary of dog, cat, or rabbit, with interstitial cells, 

 the body of which appears to be subdivided by a protoplasmic network 

 more or less restricted to small areas of round, oblong, or polygonal 

 shape. This special conformation of the interstitial cells is particu- 

 larly frequent and easy to demonstrate in the cat. It is due to the fact 

 that the cell is infiltrated with globules which stain black, not only 

 with osmic acid, but with chrom-aceto-osmic acid. The globules are 

 particularly large in the cat ; much smaller in the rabbit. They are 



* Arch Sci. Phys. et Nat., xviii. (1887) pp. 558-9. 



