458 SUMMARY or CtlKKENT BESEAEOHES EElATINa TO 



nitrS.™"°" "'"'' «'''"°' ^"^»'"«' ^1™^'' ^''°™d ae presence of 



A second addition of distilled water to the «ektin showed fUf fi. 

 watery extract was now free from nitrates ' ""* *^^ 



tl.em with a mixture of equal parts ofllnhTo a '7^^^ ^^^^ ^y covering 

 hundredth to one-thousaS orcofrosive ^i^rr' ^''''KTT 

 After 24 hours or so, the fluid is poux^d ^ r j'^^ been added, 

 be exercised in pouring the preser^Xf fl u!d o^e^ttTurflcr ^"^' 

 liquefying colonies are not at all damaged by tLpJLedurpWr T 

 mg colonies must be protected by fixin^ them downT.1' . '^''''^^- 

 glass, otherwise they run out and^eave^ht'etTa^o'nTrir' ^ '"^^'^- 



test'tXs'l^ftfr i^'ll^;^^^^^^^^^^ Cultivation t-(a) When 



moistened durin. sterlizXr&e flS,- ^^*^°^-™d plugs become 



according tu Dr sS f Z' fl'. ^^^^^^^^ence is quite avoided, 



200ccm^e.usedftSl*tr^^^^^ 



running up into the plug prevents the moisture from 



is easily withdrawn bf S p^^iX aTt'tle't f"\'''^- '^' ^^"^^ *^^^ 

 Of course, for non -aerobic or?ank^^« fl. • * "^^^^^ ^^ *« 1"««^" it- 

 it at all. 'in the latteJ^se ff'^t bTdesirrd t" Z TT'f *^ ^''""'''^ 

 the outer tube must be removed with a diamond ''^'^^' ' ^'''' "^ 



useJt'ev^Jlryl^:!^^^^^^^ ^- Schill has 



glass, which ai/aboutTcni broad 10 om 1 .^T'^TI'^ ^^^^^^^^^^ 

 U cm. apart. The neck which W. 1 ^'I^A ^^^ *^^ ^^^^^s about 



long, and is situate Tbouf he mMe^rn^. "^ M^^" '' ^^^^' ^ «-• 

 third full of gelatin and I.?d^. \ ?^f *^^ '"^^^l sides. One 

 gelatin plate 50-6? cm. square "^ '^^'' '^''' ^"^''^'^ ^^-^^ a 



Wafers for Cultivation Purposes.§— Wafers mwnf ^ 

 recommended by Dr. Schill as soTi^^^l r > (Oblaten) are especially 



II Fotlschritte d, iuClm. pp. 769I73: °' •■■ ''^''- § !■ c, p. 340. 



