462 SUMMARY OF CURRENT RESEARCHES RELATING TO 



0^^-^nto paper by moistening the reverse side of the paper by a solution 

 tl :12 nlf "^ in absolute alcohol. In the eases of 'colourless spores 

 tlie clifficul y IS to find a coloured paper the pigment of which is not 

 soluble .n alcohol ; and Dr. Harz used iLtead a sHghtly warmed solution 

 of 1 Yo, Canada balsam m 4 vols, turpentine oil, placed with a fine 

 camels hair brush on the reverse side of the paper.^ In thlcoursa of 

 from two to four days the preparation can then be laid aside between 

 paper, but is not completely dry for several weeks. 



Car tee F. B -Desmids : their Life-Mstory and their Classification II 



[Co>.tam8 directions for " Collecting '• and " Preserving andllountin; "1 



Jam.s, F. L.-Tlie Philosophy of Mo^X,%ft f '''''■'''• ^''''^^'^- ''-'■ 



Amer. Mon. Micr. Journ., X. (1889) pp. 61-3 

 W A T r F R T H- Tvr4„ 1. • , ,, ^"^^ ' Elementary Microscopical Teclinologv ' 

 vv ALLER, T. H.-Micro-chemical Methods for the Examination of Minerals 



Midi. Naturalist., XII. (1889) pp. 59-65. 



C3) Cutting-, including- Imbedding- and Microtomes 



turp^eSnf nailffi^ Paraffin.*-Dr. G A. Piersol sa3 s that although the 

 tuipentine-paiaffin so commonly employed in histolo<rical work vield« 

 excellent results, the advantages of chloroform-paraffin havlled to it 

 exclusive adoption in the laboratory of the University of Pennsylvan k 

 aid fo?;^ "''^^' *".f''"'' homogeneity of the paraffin after imbeddTn'" 

 and for this purpose the method of Kolliker is employed. In this tlfe' 



water?h '""'"^ '^' "^J^"* "^"^ ^^1*^'^ P^^^ffi^ ^^ surrounded with cold 

 Avater, the upper surface, which is alone left exposed bein^ cooled b^ 

 blowing until a film is formed, when the whole is"^ submerged^ """^"^ ^^ 

 Ihe best paraffin is that commercially known as winter worked ffum 

 stock, and comes m cakes about 4 cm. thick; that havint a bluish th^ 

 and emitting a metallic ring when struck is the best " 



mendf'deck'nW^^^^ Imbedding.t-Dr. G. C. Freeborn recom- 



S manufttoers^of ^^l^T '^^'^^/'^ ""^ ^^'' P^^^' *« ^e obtained 

 to U in T?nf Lf 1 ^''''^^' ^^^ ^^"^^ ^^ diameter from 1/2 in. 



Z r^i f 1 , ?}'^ ^""^ *^^y ^"* ^""^^ soft and yielding bv soakinl^ 

 ^llr fi^''^°^' ^"* *^^y ^^y ^^ ^^itt«« "Pon wfthleTd pencil thuf 

 oralcZl^""^^^^^^^^* *^ keep several spe'^imens in the ^0^ 



James, F. L.— Sharpening the Section Knife. 



St. Louis Med. and Surg. Journ., LVI. (1889) pp. 156-7 (2 figs.). 



C4) Staining- and Injecting-. 



Tdd v^v'slowlv ?/2 fnT"*'''/*^'"^\S *^^ ^^'1'' ^itl^ ^Vss rod, and 

 to be added I^uVn A T' ^^ ^.^^"'^ "^^^- ^^^^ ^^^^i^^* ^1"^ is 



then filter and !dd 4 oz of .".-fiT'^-^^^'^^n ^^* ''^^' ^^ ^^ t-^^' 

 use at once. ^ '^''*'^'^ 'P^'^*' This solution is ready foi- 



^ ^; University Medical Magazine, December 1888. The Microscope, ix. (1889) 



: S?:SSS?£-^^fS.^°" 'Pl^armaceutical Era.' 



