ZOOLOGY AND BOTANY, MICBOSCOPY, ETC. 465 



precipitate results which does not interfere with the staining. The 

 sections are stained in this mixture for 6 minutes, or they are stained 

 for 10 minutes in the yellow fluid, and then for 30 seconds in the 

 violet. 



Epidermis, hair-shaft, inner root-sheath, connective tissue, and 

 elastic fibres, the membrane of fat-cells, and striated muscle stain 

 yellow ; the rete Malpighii, the outer root-sheath, all glands, smooth 

 muscle, and cartilage with its cell-nuclei stain violet. 



Metanil-yellow and safranin. — Preparation: human lip, alcohol 

 hardening. 



Mix 6 ccm. of the yellow fluid with 1 ccm. of safranin. An amorphous 

 precipitate forms. This mixture gives either with a long or short stain 

 more sharp pictures than the successive single staining. 



Connective tissue stains yellow ; epidermis, the rete Malpighii and 

 the analogous layer in the mucous tissue, muscle, and labial glands stain 

 light red, the nuclei standing out sharply. 



Metanil-yellow and crystal Ponceau [alphaazonaphthallindisulfo- 

 betanaphthol of soda]. — Preparation : spinal cord of calf, alcohol 

 hardening. 



For the single as well as tlie combined stain, 24 hours are required. 



The grey matter stains yellow, the white reddish. Under strong 

 magnification, the neuroglia and connective tissue are found to be stained 

 yellow ; the axis cylinders dark bluish-red ; the myelin light yellowish- 

 red ; one sort of ganglion-cells dark purple, another bluish-red ; nuclei 

 do not stand out sharp. 



Metanil-yellow and Congo-red [tetraazodiphenyldinaphthylamindi- 

 sulphonate of soda].— Preparation : spinal cord of calf, alcohol hardening. 



The sections are stained in a mixture of the staining fluids for eight 

 minutes, or they are stained for ten minutes in the yellow stain and then 

 for five minutes in the red. 



Ganglion-cells [without clear nuclei staining] and axis-cylinders 

 stain dark violet-red ; the medullary sheath light citron-yellow ; 

 neuroglia and all connective tissue light violet-red ; epithelium of the 

 central canal brownish-red. 



Carminate of soda and metanil-yellow. — The central nervous system 

 is hardened in Miiller's fluid, then stained in toto with the carmine fluid. 

 Sections are then stained for ten minutes in the yellow stain. 



All nervous elements are stained red ; all connective tissue elements 

 yellow. 



Crystal Ponceau and crystal violet. — Preparation : transverse section 

 of the carotid of the calf, alcohol hardening. 



The sections are stained for five minutes in the red Ponceau fluid, 

 and then for one minute in the violet. 



Nuclei of the endothelium and smooth muscle stain violet ; all the 

 other tissues red. 



Congo-red and ani sol-red [bisulfoxylnatronbetaoxynaphthalinazo- 

 orthometoxylbenzol]. — Preparation : spinal cord of the calf, alcohol 

 hardening. 



The sections are stained for five minutes in the combined stains, or 

 for five minutes in the Congo-red solution and then for five minutes in 

 the anisol-red. 



Axis-cylinders and cell-bodies stain purple ; all other tissues stain 

 light red. Nuclei do not stain. 



