466 SUMMARY OF CURRENT RESEARCHES RELATING TO 



Metanil-yellow and ethylin-blue. — Preparation : ala nasi of a child, 

 alcohol hardening. 



When the two staining solutions are combined a black precipitate is 

 formed, which redissolves in an excess of the metanil-yellow solution. 

 This solution stains yellowish-green, the cartilage only being stained 

 blue. If the sections are first stained for five minutes in a mixture of 

 5 ccm. of the yellow and 4 ccm. of the blue stain, or if the sections 

 are stained for ten minutes in the yellow and then for two minutes in the 

 blue, the pictures will be sharp. 



The epidermis, hair-shaft, outer root-sheath, connective tissue, elastic 

 fibres, smooth and striated muscle stain yellow ; all glands, membrane 

 of fat-cells, cartilage, and nuclei stain blue. 



Triple stains. — Metanil-yellow, methyl- green, and safranin. — Pre- 

 paration : ali nasi of a child, alcohol hardening. 



The sections are stained for eight minutes in the yellow solution, 

 then for thirty seconds in the safranin solution, then for twenty seconds 

 in the methyl-green solution, and finally passed through the metanil- 

 yellow solution. 



The different elements are differentiated as in the double stain with 

 metanil-yellow and methyl-green, except the colour is of a darker shade, 

 and all muscular elements are stuined red. 



Metanil-yellow, crystal Ponceau, and crystal violet. ^ — Preparation: 

 ala nasi of a child, alcohol hardening. 



The sections are stained for 2-16 minutes in a mixture of 5 ccm. of 

 the yellow solution, 5 ccm. of the Ponceau solution, and 3 ccm. of the violet 

 solution, or they are stained for eight minutes in the yellow solution, 

 then for six minutes in the Ponceau solution, and finally for fifteen seconds 

 in the violet solution. 



Cartilage and nuclei of cartilage-cells, the superficial layer of the 

 epidermis stain bluish-violet ; connective tissue, elastic fibres, and 

 glands stain light red ; the deep layer of the epidermis, the rete 

 Malpigbii, hair-shaft, the root-sheaths, membrane of fat-cells and muscle 

 stain yellow. 



Metanil-yellow, azo-blue, and methyl-green. — Preparation : ala nasi 

 of a child, alcohol hardening. 



The sections are stained for ten minutes in the yellow solution, then 

 for six minutes in the blue solution, and then for two minutes in the 

 green solution, finally, the sections are passed through the yellow 

 solution. 



The epidermis, hair-shaft, inner root-sheath, smooth and striated 

 muscle stain yellow ; membrane of cells, the rete Malpighii, membrana 

 propria of glands, elastic fibres, and connective tissue stain violet ; nuclei 

 of gland cells and nuclei of the cells of the Malpighian layer, outer root- 

 sheath, smooth muscle, and connective tissue stain green. 



Crystal Ponceau, methyl-green, and crystal violet.— Preparation : ala 

 nasi of a child, alcohol hardening. 



The sections are stained for eight minutes in a mixture of 10 ccm. of 

 the Ponceau solution, 4 ccm. of the green, and 2 ccm. of the violet, or they 

 are stained for eight minutes in Ponceau solution, then for three minutes 

 in the methyl-green solution, and then for five seconds in the violet 

 solution. 



The epidermis, hair-shaft, and outer root-sheath stain violet ; smooth 

 and striated muscle, elastic fibres, and connective tissue stain rose-red ; 



