468 SUMMARY OF CUBEENT KESE ARCHES RELATING TO 



tion, but the author specially recommends phosphoric acid and chloride 

 of calcium as being likely to replace advantageously iodized chloride of 

 zinc and iodized sulphuric acid. 



The author then describes the preparation of several of these new 

 reagents. In order to make iodized phosphoric acid, the pure crystal- 

 lized phosphoric acid must be taken, and to this must be added, in order 

 to effect solution, a fourth or a third of its bulk of water, and then some 

 crystals of iodide of potassium or iodine must be added until the liquid 

 acquires the tint of rum or curagoa. It is advisable to prepare this 

 reagent in different states of concentration. It will be found to colour 

 cellulose in a few minutes a deep blue colour. Occasionally, when the 

 cellulose coloration is found to be partly masked by other matters 

 present, it may be advisable to warm the sections to be studied with 

 a weak solution of hydrochloric acid (1 per cent.) or potash (4 per cent.). 

 After this the staining will be found to appear instantly. 



Staining the Bacillus of Glanders.* — Dr. H. Kiihne, who considers 

 the staining of B. mallei to be especially difficult, advises the following 

 procedure. Before immersing in the stain the sections are to be 

 thoroughly freed from spirit. This done, they are placed for 3-4 minutes 

 in carbol-methylen-blue (water, 100 ; carbolic acid, 5 ; alcohol, 10 ; 

 raethylen-blue, 1 • 5 gr.) and then decolorized in water acidulated with 

 hydrochloric acid, after which the acid is extracted with distilled 

 water. After a transitory immersion in alcohol they are transferred to 

 anilin oil to which 6-8 drops of oil of turpentine have been added. 

 Then to pure turpentine, xylols and lastly balsam. 



New Rapid Process for Staining Bacillus Tuberculi. — MM. Pittion 

 and Roux have presented to the Societe de Medecine de Lyon f a process 

 for differential staining of bacillus tuberculi, in which the easily decom- 

 posable anilin water, or its substitute, carbolized water, is supplanted by 

 aqua ammonige. There are in the process three fluids, viz. : — 



Solution A. Ten parts of fuchsin dissolved in 100 parts of absolute 

 alcohol. 



Solution B. Three parts of liquid ammonia dissolved in 100 parts 

 of distilled water. 



Solution 0. Alcohol, 50 parts ; water, 30 parts ; nitric acid, 20 parts ; 

 anilin-green, to saturation. In preparing this solution dissolve the 

 green in the alcohol, add the water, and lastly the acid. 



To use. To 10 parts of solution B add 1 part of solution A, and 

 heat until vapour begins to show itself, then immerse the cover-glass, 

 prepared as in the ordinary method of staining. One minute suffices 

 to thoroughly stain the bacilli. Wash with plenty of water, and after 

 rinsing with distilled water let fall on the film side of the cover-glass 

 2 or 3 drops of the green solution (C), and let it remain not longer than 

 40 seconds. Wash off with abundant water, dry, and mount in xylol 

 balsam. The bacilli will, on examination, be found to be stained a fine 

 rose red upon a pale or delicate green ground. 



Most excellent preparations may be obtained by replacing tbe 

 fuchsin with gentian-violet and the anilm-green with a weak solution 

 of chrysoidin. 



An experiment made with the above stain by Dr. F. L. James J seems 



• Fortschritte der Med., 1888, p. 860. 



t St. Louis Med. and Surg. Journ., Ivi. (1889) p 155. X T. c, p. 156. 



