ZOOLOGY AND BOTANY, MICEOSCOPY, ETC. 845 



to the slide and tte covers to the cell, by means of heat alone, or by 

 varnish. A thin or thick object can be mounted in the same cell by 

 placing the cover either upon the shoulder or upon the rim of the 

 cell, and last, and not least, to those who have large exchange lists, 

 is their inexpensiveness, as they can be made for less than two cents 

 per dozen. 



Fluid for Mounting Infusoria, Algae, &c.*— Dr. T. F. Allan 

 uses the following solution for mounting Infusoria, Algte, Characese 

 &c., which it is said preserves the arrangement of the cell-contents in 

 a most excellent condition. 



Wood-vinegar, sp. gr. I'Od, 100 parts. 

 Salicylic acid, 1 part. 



Shake and allow to settle. This mixture is named " salicylic 

 vinegar." 



For Algae, mix salicylic vinegar 1 part, glycerin 1 part, water 

 20 parts. 



For Infusoria, mix salicylic vinegar 1 part, glycerin 10 parts, 

 water 40 parts. 



Preservative Fluids for Botanical Preparations.f — In 1872 

 MM. J. Greenland, M. Oornu, and G. Eivet published a little work J 

 on the preparation of Botanical objects for the Microscope, in which 

 they gave a series of formulae which they had found useful. This 

 work being now rare, M. G. Huberson has reproduced these formula 

 as follows : — 



No. 1. Equal volumes of glycerin (as pure as possible), alcohol, 

 and camphor-water. This fluid will preserve, either in bottles or 

 cells, the greater number of vegetable tissues, and especially all 

 cellular tissues of a certain solidity, such as horny albumen, epidermis, 

 sections of leaves, and all woody, fibrous, and vascular tissues. 

 Certain preparations it renders too transparent, especially those of 

 very young organs ; a fault that is corrected by the addition of water. 

 (For tissues with cystoliths Nos. 3, 4, and 5 are preferable.) 



No. 2. Three volumes of glycerin to two of camphor-water may be 

 put to the same use as No, 1, but only in closed cells. 



No. 3. 100 grammes of distilled water to two of chloroform. Shake 

 up together for five minutes at least. About 1 gramme of the chloro- 

 form is dissolved, the remainder is precipitated at the bottom, and 

 serves to keep the fluid saturated. It is used for all tissues in course 

 of development and still tender, prothallia, embryonal sacs, arche- 

 gonia and the fecundating organs of cryptogams in course of forma- 

 tion, (For the same parts completely developed Nos. 1 and, 2 are 

 preferable.) 



No. 4. The same as No. 3, with the addition of 4: or 5 grammes of 



* Amer. Mon. Micr. Journ. ii. (1881) p. 98. 



t Brebissonia, iii. (1881) pp. 104-8. 



X Des preparations microscopiques tirees du regne vegetal et des difife'rents 

 proce'de's a employer pour en assurer la conservation, (8vo, Paris, 1 872.) 76 pp. 

 and figs. 



Ser. 2.— Vol. I. 3 K 



