II. OBSERVATIONS ON TRICHOGLGEA LUBRICA. 



Fred. K. Butters. 



The specimens of Trichogloea lubrica (Harv.) J. Ag., upon 

 which the following observations were made, were collected by- 

 Miss Josephine E. Tilden, June 13, 1900, at Kahuku point, at the 

 northwest extremity of the island of Oahu, Hawaiian Islands, 

 from material which had been cast up on the beach. Specimens 

 from the same collection were subsequently distributed by the 

 collector (American Algae, Century V., No. 419) as Nemalion 

 ramolusum Harv. 



Several entire plants were preserved in a 1 per cent, solution 

 of formaldehyde, and a small amount of material was killed in 

 1 per cent, chromic acid, and then, after thoroughly washing, 

 transferred to 70 per cent, alcohol in which it was preserved. 

 Material treated according to both of these methods was used 

 in preparing the present paper. For most purposes the formal- 

 dehyde material proved more useful, as by this method the gela- 

 tinous matrix of the frond was preserved more nearly in its 

 natural condition. In studying the microscopic anatomy it was 

 found that many points could be best made out by picking to 

 pieces portions of the frond with needles, or by crushing por- 

 tions under the cover glass. The latter process was aided 

 by the gelatinous nature of the frond. Besides preparations 

 made by dissecting and crushing the frond, sections were made 

 by various methods. For the study of the anatomy of the vege- 

 tative tract it was found that the best preparations were obtained 

 by imbedding the material in celloidin, hardening the block 

 with chloroform vapor, and cutting in the usual manner upon 

 the sliding microtome. Sections were thus obtained in which 

 the loose cortical structures were held in their proper positions. 

 Useful preparations were made by mounting the material on a 

 freezing chamber in a drop of gum arabic solution and cutting 

 the frozen mass on the sliding microtome. This material did 



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