708 SUMMARY OF CURRENT RESEARCHES RELATING TO 



The following items from the catalogue will give some idea of the 

 objects prepared : — 



3. Orchid leaf. Fibro-spiral cells. 

 19. Yew. Isolated wood cells. 

 27. Brake fern. Scalariform vessels. 

 53. Pampas grass. Closed vascular bundles. 

 75. Mistletoe. Thickened cuticle cells. 

 99. Eucalyptus. Oil glands in leaf. 

 184. Begonia. Axile placentation. 



In many cases the objects have been stained, either singly or doubly, 

 and some stained three years ago have not faded. Their very low cost 

 commends them to every student of biology or collector of microscopic 

 objects. 



They may be mounted in either resinous media (dammar, benzol- 

 balsam), or glycerin or glycerin-jelly. Mr. White's instructions for 

 mounting are as follows : — " Carefully separate the inclosing films, and 

 remove the object. If for resinous media, soak in spirit of turpentine 

 till clear, rinse in a fresh portion of the same, then drain, transfer to 

 the cover or slide, and finish in the usual way. For glycerin : — If the 

 object be oily, first wash out the oil with strong methylated spirit till 

 clear, transfer to a mixture of glycerin and water, equal parts, in which 

 let it remain an hour or two, then mount. 



" Minute objects, such as isolated cells, should be transferred on the 

 point of a scalpel to a slide (or cover), and separated with a needle in a 

 drop of spirit ; then, if for glycerin, mount while still moist ; but if for 

 resinous media, allow to dry, then moisten with a drop of turpentine 

 before applying the medium. Spiral and other vessels, and long fibre 

 cells, which mat together, should be soaked in a drop of weak spirit, and 

 a few of the most perfect picked out under a simple lens." 



Bacteriological Technique.* — Dr. C. Giinther suggests that agar 

 plate cultivations may be preserved on slides by cutting out a thin layer 

 and then imbedding in glycerin. The specimen is to be mounted on the 

 slide in the usual way. 



The author also suggests that the condensation water of potato culti- 

 vation in test-tubes may be prevented from coming in contact with the 

 potato by resting the latter upon a piece of glass tube about 2 cm. long. 

 The latter lies on the bottom of the test-tube. In other respects the 

 author advises Hueppe's technique. 



Beck, J. D. — A Slide of Hints and Suggestions. 



[Cleaning slides — How to dispose of excess of media on slides — Clipping 

 covers — Centering and ringing clipped covers — Cements — Final cleaning of 

 slides — Double-staining animal tissues — Pale copal varnisli — Black elastic 

 varnish — Aluminium palmitate copal varnish.] 



The Microscope, IX. (1889) pp. 205-12. 

 Oanfield, W. B. — On the Microscopical Examination of Urinary Sediment. 



Queen's Micr. Bulletin, VI. (1889) p. 26. 

 Chadwick, H. — Mounting Insects in Balsam without pressure. 



Queen's Micr. Bulletin, VI. (1889) pp. 31-2. 

 DuFOUE, L. — Revue des travaux relatifs aux Methodes de Technique publics en 

 1888 et jusqu'en avril 1889. (Review of the works relating to methods of 

 Technique published in 1888 and down to April 1889.) 



[1. Methods of preservation and culture. 2. Processes for treating the 

 sections. 3. Microscopy. 4. Photomicrography. 5. Various.] 



Revue Gen. de Botanique, I. (1889) pp. 280-92, 343-56 (4 figs.). 



* Deutsch. Med. Wochensclir., 1889, No. 20. 



