ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 669 



p. Technique.* 

 CI) Collecting' Objects, including' Culture Processes. 



Blood-serum Cultivation.t — Dr. F. Hueppe combines the advantages 

 of blood-serum for growing micro-organisms with the advantages of plate 

 cultivation for separating the colonies in the following manner: — Blood- 

 serum is sterilized at a temperature of 58'-60^ by the discontinuous 

 method. It may, however, be sterilized at once and with safety by heating 

 to boiling-point, but although its nutritive properties are apparently 

 unaffected, it loses slightly in transparency. The author gives an illus- 

 tration of a modification of Fol's sterilizer, heated by the same arrange- 

 ment as the author's own thermostat.J The tubes are laid in the oblique 

 position. After sterilization the serum is warmed to 37*^ C. and inoculated 

 in the usual manner. 



Meanwhile, a 2 per cent, agar solution, to which • 5-1 per cent, grape 

 sugar is added, has been prepared. Having been fluidified, the agar is 

 cooled down to 42°-45°. Equal quantities of the warm inoculated blood- 

 serum and of the warm agar solution are then mixed together, with the 

 usual precautions, and having been well shaken up, are allowed to solidify 

 in plates, bulbs, &c., at the ordinary temperature. When firm the cultiva- 

 tions are removed to the thermostat. By this method the breeding of 

 tubercle-bacilli from sputum succeeds pretty well, 



C R s I E E, R. — A method of inoculating fluid cultivating media. 



Brit. Med. Joum., 1886, No. 1347, p. 769. 



Edington, a. — A ne'w culture medium for micro-organisms capable of withstanding 



high pressure. Lancet, 1886, II. p. 704. 



Griessmayee.— Die Eeinkultur der Microben mit specieller Kticksicht auf die Hefe. 



(The pure culture of microbes with special reference to yeast.) 



Allg. Brauer- und Hopfen Ztg., 1887, pp. 591-2, 603-5. 

 KoLKSSNiKOw.— See Tarchanow. 



Mace. — Sur la preparation des milieux a la gelose pour la culture des bacteries. (On 

 the preparation of gelatin media for the cultivation of bacteria.) 



Ann. Inst it. Pasteur, 1887, pp. 189-90. 

 Smith, T. — The relative value of cultures in liquid and solid media in the diagnosis 

 of bacteria. Med. Kevs, 1886, II. p. 571. 



Sternberg, G. M. — Bacteriological Notes. The liquefaction of gelatin by bacteria. 



Med. Aews, 1887, pp. 372-3. 



Tarchanow and Kolessnikow. — Die Anwendung des alkalisch gemachten 



Eiweisses von Hiihnereiem als durchsichtiges Substrat zur Kultiu- der Bacterien. 



(The use of alkaline albumen of hens' eggs as a transparent substratum for the 



culture of bacteria.) Uusskaja Medicina, 1887, No. 11 (Russian). 



Terry, W. A. — Notes on Diatom study. 



[Dredging for diatoms.] Amer. Mon. Micr. Joum., VIII. (1887) pp. 44-6. 



ViGNAL, W. — Etuve pour Cultures. (Culture oven.) 



Ann. Instit. Pasteur, 1887, pp. 184-8. 



(2) Preparing Objects. 



Method for subjecting Living Protoplasm to the action of different 

 liquids. § — Mr. G. L. Goodall, for studying the action of very dilute solu- 

 tions on living protoplasm, obviates the necessity of transferring the 

 si^ecimen from the litre-flask, as in the methods of Loew, Bokorny, and 

 Pfeffer, to the stage of the Microscope, by using an apparatus consisting 



* This subdivision contains (1) Collecting Objects, including Culture Processes ; 

 (2) Preparing Objects; (3) Cutting, including Imbedding and Microtomes; (4) Staining 

 and Injecting ; (.5) Mounting, including slides, preservative fluids, &c. ; (6) Miscella- 

 neous. 



t Centralbl. f. Bacteriol. u. Parasitenk , i. (1887) pp. 607-10 (1 fig.). 



I Med. Wochenschr., 1886, No. 17. 



§ Amer. Jouru. Sci., xxxiii. (1887) pp. 144-5. 



