690 SUMMARY OF CURRENT RESEARCHES RELATING TO 



The plates are placed on blotting-paper and dried rapidly. They only 

 keep for a few days. 



For preparations stained with vesuvin, Bismarck brown, methyl-green, 

 or picro-carmine with yellow and red stain, the formula which gives the 

 best results is that of Mulnian and Scolik : — 1 gr. of quinoline red is 

 dissolved in 500 cc. alcohol, and 50 cc. of an alcoholic solution of cyanin 

 1 : 500 is added. The plate is steeped for a minute in water 100 cc. ; am- 

 monia 1/2 cc. It is then transferred to a bath composed of the quinoline red 

 solution 1 cc, ; water 100 cc. ; ammonia 1/2 cc, for one minute. The super- 

 fluous water having been removed with blotting-paper, the plate is dried in 

 a stove at about 30'^. 



For preparations stained with any colour the following formula succeeds 

 well: — Bath for two minutes in a watery solution of erythrdsin 1:1000, 

 25 cc. ; ammonia 4 cc. ; water 175 cc. If the preparations are stained red, 

 1 cc. of an alcoholic solution of cyanin 1 : 500 is added. 



Another formula is — Solution of erythrosin 1 : 1000, 25 cc. ; solution of 

 silver nitrate 1 : 1000, 25 cc. ; water 50-100 cc. ; and if the preparations are 

 deeply stained with red, the author adds 5-10 cc. of an alcoholic solution of 

 cyanin 1 : 500. 



Dr. Francotte remarks that it is absolutely indispensable to use ortho- 

 chromatic plates when dealing with coloured preparations, and if the stain 

 be blue or violet, a yellow glass must be interposed between the light and 

 the preparation. 



For developing, the author prefers pyrogallic acid and sulphite of soda. 

 Four baths are required : — (1) 10 gr. pyrogallic acid dissolved in 100 cc. of 

 alcohol at 90°. (2) 100 gr. of pure sulphite of soda dissolved in 200 cc. 

 distilled water. (8) 100 gr. of pure carbonate of soda dissolved in 200 cc. 

 distilled water. (4) An aqueous 10 per cent, solution of bromide of potash. 



In order to develope, 5 cc. of No. 1, 10 cc. of No. 2, and 5 cc. of No. 3 

 are poured into a vessel containing 100 cc. of water, and if the time of ex- 

 posure be in excess, a few drops of No. 4 are added. 



The time of development is about five minutes. 



Fixing is performed in the usual way. If the plates are still coloured 

 after the operation (and this often happens) they are immersed in a bath of 

 spirit at 90°, to which a few drops of ammonia are added. 



BiDEET. — Ein Verfahren, den Nachweis vereinzelter Tuberkelbacillen zu sicliern, nebst 

 Bemerkungen iiber die Farbbarkeit der Bacillen und Aetiologie der Tuberculose. 

 (A process of authenticating the presence of single tubercle bacilli, with remarks on 

 the staining capacity of the bacilli and the aetiology of tuberculosis.) 



Berl. Klin. Wochenschr., 1886, Nos. 42, 43. 

 Cf. CentralU. f. BacterioL, I. (1887) p. 55, 

 Dekhuyzen, M. C. — Ueber die Tinction. (On staining.) 



Centrum, f. d. Med. Wiss., 1886, Nos. 51-2. 

 DoHBRTY, A. J. — The Staining of Animal and Vegetable Tissues. 



[" Tlie object of the present paper, which is addressed to professed biologists as 

 well as to dilettanti, is twofold ; firstly, to record the results of my own exten- 

 sive researches into the properties of staining reagents; and secondly, to place 

 before the microtumist in a condensed form an account of various processes 

 adopted by other workers with the Microscope."] 



Trans, and Ann. Rep. 3Ianchester Micr. Soc, 1886, pp. 1-19. 

 Gbigeejew, a. — [On Ehrlieh's Staining of Micro-organisms.] 



Russkaja Med., 1886, No, 42. 

 Herxheimee, C. — Ein neues Farbungsverfahren fiir die elastischen Fasern der 

 Haut. (A new staining process for the elastic fibres of the skin.) 



Fortschr. d. Med., IV. (1886) p. 787. 

 Kamenski, D, a. — Eine neue Methode die Koch'schen Bacillen im Sputum zu farben. 

 (A new method of staining Koch's bacilli in sputum.) 



Wratsch, 1887, pp. 276-7 (in Russian). 



