330 Transactions of the Society. 



the growth. No further growth took place during the eight days 

 in which the preparations were under observation. 



Experiment 3. Cell cultivation with Gelatine peptone. — 

 Gelatine peptone, as suggested by Koch, but with a larger portion 

 of gelatine, was used in the place of aqueous and vitreous humours, 

 as being more easily obtained, sterilized, and more uniformly 

 solid. A small portion of the jelly was cut out and placed on a 

 cover-glass with a ringworm hair on its surface. The cover-glass 

 was then inverted so as to form the roof of a putty cell, air being 

 admitted by a narrow passage. Two preparations were put up in 

 this way and two closed. After 24 hours in the incubator at 

 23° C. spores were seen to be swollen in all the specimens, and in 

 the closed cells they had begun to develop buds. After 72 hours 

 these buds had elongated into distinct mycelial filaments, which 

 were longest towards the end of each hair where the jelly had 

 accumulated. The open specimens contained micrococci and 

 bacteria, and had shown no further sign of growth. On the sixth 

 day the mycelium had extended into long filaments, showing septa 

 in places. 



Experiment 4. — Six more specimens were mounted, all in 

 closed cells as before. Swelling and budding took place within 

 48 hours. On the third day mycelial growth was distinct in four. 

 One specimen had dried up ; in the other, bacteria had developed 

 and obscured the spores. 



The mycelium became septate during the next four days, but 

 as the fluid gradually evaporated, growth ceased, and by the ninth 

 day all were dried up. 



Experiment 5. — Six more specimens were prepared in the 

 same manner. Growth took place in all within 72 hours. On the 

 fourth day oil from the putty had mixed with the fluid of the jelly 

 in three, while the remaining three had dried up. 



Experiment 6. — At this point putty cells were given up and 

 hollow glass cells, circular and oval, were used instead. 



Hairs from the same case were placed at the bottom of the cell 

 and completely covered with the gelatine peptone. Cell was closed 

 with a cover-glass. Three specimens were placed in the incubator 

 at the temperature of 23° 0. In 24 hours the spores had swollen 

 and begun to bud. In 48 hours distinct filaments were growing 

 from sides of the hairs, chiefly towards the ends. Specimens were 

 then lett at room temperature, and on the fourth day filaments had 

 grown twice the length, and showed septa. Isolated spores, which 

 had been separated from the hair, had also produced filaments. On 

 the sixth day contents of filaments were becoming granular, and 

 highly refractile spaces began to appear near the ends, in the midst 

 of the protoplasm. In places two filaments or more were seen 

 arising from one spore. The protoplasm next became aggregated 

 in the centre of the filaments, and further growth ceased. Frag- 



