Ringworm Fungus. By M. Morris & G. C. Henderson. 331 



ments of jelly containing these bodies were transferred to fresh 

 slides and gelatine peptone, but no growth took place from them. 



Experiment 7. — Ten specimens were prepared in a similar 

 manner to the last mentioned, with uniform results in every case. 

 Growth ceased before formation of definite fructifying organs. 



Experiment 8. — Seven new specimens from a fresh case of 

 typical ringworm were prepared as before and placed in the 

 incubator at 24° C. After 24 hours spores were pear-shaped. In 

 these specimens several isolated spores were watched from day to 

 day. In 48 hours the buds had elongated into filaments, no 

 bacteria or adventitious fungus having appeared in the meantime. 

 In three of the specimens growth ceased on the sixth day, but in 

 the remaining four, which had a less amount of peptone, growth 

 extended to the margin of the jelly, branching freely, and finally 

 sending oflT twigs at an angle of 45°. Some filaments presented 

 pear-shaped enlargements at their ends. During the next three 

 days these enlargements underwent no further change, but the other 

 terminal filaments commenced to form basidia, sterigmata, and 

 chains of spores in a manner similar to PeniciiUum. 



Some of the spores, when removed to fresh gelatine peptone, 

 grew into exactly the same forms of filaments and fructification. 

 They also produced when placed on the human skin beneath a 

 watch-glass fixed by plaster, a crop of itching papules on the third 

 day, and these about the sixth day coalesced to form an erythe- 

 matous patch, the centre of which gradually faded and desquamated, 

 while the margin spread centrifugally, like a typical patch of ring- 

 worm. After washing the surface, scales of epidermis were removed 

 from the margin, and on soaking in liquor potassse, were found to 

 contain numerous spores, identical in appearance with Trichophyton 

 tonsurans. 



The second generation of spores also produced a typical patch 

 of ringworm, which contained fungus. 



Experiment 9. Cultivation in tubes. — Twelve tubes, six with 

 hairs at the bottom of the tube, six with hairs floating on the 

 surface of the gelatine peptone. The tubes had been sterilized 

 with care by heating in the flame of a spirit-lamp to dull redness 

 and then plugged with wool. In 24 hours spores in both sets 

 were swollen, pear-shaped, and some had short filaments. During 

 the next day mycelial growth continued. On the sixth day the 

 hairs on the surface had begun to throw up whitish aerial hyphas, 

 which in two days developed abundant spores. 



The spores on the submerged hairs produced very long fila- 

 ments, the protoplasm of which began to become granular and 

 aggregated in places about the seventh day, then ceasmg to grow. 



Healthy hairs submerged and floating in the same way showed 

 no results. 



Experiment 10. — Spores were removed from the fructifying 



