336 Transactions of the Society. 



hyphse and mycelium become vacuolated, sporangia show " aggre- 

 gations of protoplasm, the future spores, and occasionally bud." 

 Sporangia were most frequent in under-fed cultivations. 



Atkinson believes Trichophyton to be a Mucor, presenting some 

 differences from Mucor 7nucedo. 



'J'hin* used cells, the hair being placed on under surface of the 

 cover-glass and a drop of fluid placed over it, sometimes so as to 

 cover it, at others only to moisten it. To prevent evaporation a ring 

 of damp blotting-paper was put at the bottom of the cell, which 

 was then kept in an incubator at 92° F. to 98° F. (33-3° C. to 

 36-6° C), most usually 96° F. to 98° F. (35-4° to 36- 6' C). 

 He also carried out mass cultivations in protected flasks, on the 

 surface and in the deej). No mention made of sterilization. 



Aqueous and vitreous humour, the latter chiefly in successful 

 experiments. Several other fluids were used but mth no success. 



In cells, spores elongated after a few hours and formed mycelium 

 during two following days, which ceased to grow after having 

 attained a very moderate length, spore formation soon taking place. 

 In flasks, similar results. Bacteria appeared in all cases, adventitious 

 fungi frequently avoided (in 9 out of 12). 



Experiments. — Three cells, twelve flasks, in eight of which 

 TrichojjJiyton developed. In one case, after being at room tempera- 

 ture in flask from April 20 to May 3rd, it had grown only as much 

 as two days in the incubator. "Growth observed consisted in a 

 development of mycelium from spores, and in the formation of 

 spores within the mycelium, as is portrayed in the drawings. No 

 organs of fructification were observed." Hairs which had been 

 immersed in water for sis days, or which had been submerged in 

 vitreous humour showed no sign of growth. Thin's conclusions are, 

 i. That Trichophyton is not one of the common fungi, ii. That it 

 can be cultivated artificially when moistened with vitreous humour, 

 iii. When covered with vitreous humour it does not grow. 



Eemakks. 



In comparing the above-described experiments, we notice that 

 Neumann's method, though giving results which coincide with 

 our own, is open to the objection that adventitious fungi could 

 find their way by means of the air to the medium in which the 

 cultivation was then taking place, as his cells were not closed and 

 had to be frequently removed from the incubator for purposes of 

 observation. The long interval which elapsed before growth took 

 place also rendered it more probable that the various fungi observed 

 by him were adventitious. 



To Grawitz we are indebted for the idea of using gelatine as 

 one of the constituents of our medium. The difference between 

 * Proc. Koy. Soc, 1881. 



