; Chemical examination of the Hop. 308 
extract. Two ounces of proof spirit were added to the 
same lupulin, and subjected to a moderate heat twelve 
hours; when filtered and evaporated, there remained six 
grains of a resinous extract. ‘The same lupulin was digest- 
ed thirty minutes in boiling alcohol, from which was ob- 
tained by evaporation sixty-two grains of extract. The 
extract obtained by the second process was soluble in pure 
alcohol, and when water was added to the solution, it be- 
came turbid and milky. 
Exp. 6.—The lupulin used in the last experiment, was 
boiled j in strong caustic ammonia. When filtered and su- 
persaturated with distilled vinegar, a copious precipitate 
ensued, which was insoluble in alcohol, and possessed the 
sensible properties of an impure wax. ‘The three last ex- 
periments show pretty satisfactorily, that the most impor- 
tant proximate principles of the lupulin are resin, wax, 
tannin, gallic acid, a bitter principle, and an extractive mat- 
ter. | ‘The following experiments were instituted for the 
purpose of ascertaining more accurately their respective 
proportions, as well as the aggregate amount of soluble 
matter in a given quantity of lupulin. 
Exp. 7th—Two drachms of lupulin were infused five 
hours in boiling water. To the filtrated infusion, were ad- 
ded at intervals, five grains.of animal gelatin in solution; 
when it ceased to produce any precipitate, and the super- 
natant liquor became transparent andclear. ‘The sediment, 
when dry, weighed ten grains. An ounce of alcohol was 
added to the filtered solution, but it caused no change 3 by ~ 
evaporation, it yielded fifteen grains of a very bitter extract. 
The same lupulin was digested again in boiling water—an- 
imal gelatin added to the filtered solution, induced no pre- 
cipitate ; ; by evaporation, an additional quantity of six 
grains of the watery extract was obtained. 
Exp. 8th.—The extract obtained in the last experiment, 
was put into pure alcohol, and frequently agitated. After 
twenty-four hours it was filtered : ten grains had been re- 
dissolved by the alcohol, and an insoluble mass 3, Weighing 
eleven grains, was left upon the filter. 
Exp. 9th.—The same lupulin which was used in the 
seventh experiment, was now digested in alcohol. The in- 
fusion was highly bitter, and ofa fine yellow colour ; it gave 
by evaporation twenty-four grains of resin. By digesting 
