306 Chemical examination of the Hop. 
in a second portion of alcohol, twelve grains more of resin 
were obtained, less bitter, but ‘otherwise like the laste:i pigs 
Exp. 10th.—The lupulin which was the subject of the 
last experiment, after having been thus boiled in water, and 
digested in alcohol, was put into a small retort, and boiled 
in two ounces of ether. While boiling, it was filtered into 
a vessel contaiming cold water, by which means twelve 
grains of wax were obtained.* 
Exp. 11th.—Half an ounce of lupulin was boiled suc- 
cessively in water, alcohol and ether. On weighing the in- 
soluble residuum, it was found that five eighths of the whole 
had been taken up by the solvents. 
From the foregoing experiments, all of which were, with 
some variation, frequently repeated, I infer, that the lupu- 
lin contains a very subtle aroma, which is yielded to water 
and to alcohol, and which is rapidly dissipated by a high 
heat; that no essential oil ean be detected by distillation in 
any portion of the hop; that the lupulin contains an ex- 
tractive matter, which is soluble only in water ; that it con- 
tains tannin, gallic acid, and a bitter principle, which are 
soluble in water, and in alcohol; that it contains resin, 
which is dissolved by alcohol and by ether, and wax, which 
is soluble only in alkalies and in boiling ether; that it con- 
tains neither mucilage, gum, nor gum- resin ; that the aro- 
matic and bitter properties of the lupulin are more readily 
and completely imbibed by alcohol than by water, and much 
sooner by both when they are hot than when they are cold; 
that about five-eighths of the whole substance is soluble in 
water, alcohol and ether, there being about three-eighths of 
it vegetable fibrous matter. These proximate principles 
exist in very nearly the following proportions :——In two 
drachms (or one hundred and twenty grains) of lupulin, 
there is, 
* The usual method of separating wax from vegetables, by boiling them 
in caustic Ammonia, and then suscr-saturating the alkali with vinegar, or 
with dilated sutpburic acid, is tedious, and the results Myst icici The 
following is a much more easy and beautiful pee? ss. After digesting the 
substance in boiling water and cold alkohol, let-it be boiled in ether, and 
the solution strained, while SMS info cold water. The wax, which is 
beld in solution by boiling ether, is thrown down as soon as the ether is 
cooled by the water, and. its specific gravity being greater than that of 
ether, and less than that of water, it forms a beautiful partifion between 
them. If the ether be suilered to evaporate, the wax may be taken irom 
the wateréntire. 
