294. Journal of the Asiatic Society of Bengal. [July, 1906, 
rotation, molecular weight and behaviour with Fehling’s solution it 
is identical bed lactose and different from the ‘tewfikose ’ described 
ichmond a 1 
Details of the isolation and examination of sugar—The milk 
used for this purpose was obtained from a buffalo in the village of 
Shibpur in May 1906.* The method adopted for isolation was iden- 
tical with that employed by Richmond and Pappel (Joc. cit.), viz., 
precipitation of the proteids and fat by mercuric nitrate solution 
(Wiley’s reagent) neutralising the filtrate with aqueous potash an and 
passing sulphuretted hydrogen gas to prec epstete: m mercury salts, 
filteri 
sugar erystallise out. It was found necessary to wash with ool 
dilute alcohol. The purification from traces of oe of the 
sugar obtained in this way was somewhat troubles 
Optical rotation was determined in aqueous selitiois 
10 per cent. boiled solution of the oy leas in a 200 1 mm. tube 
gave a — 4 10°30’, 
Found. For lactose in © 
10 per cent. 
| solution. 
[a], 52°30’ 52°30’. 
_ Molecular Sioa was determined by the freezing point method. 
0°4670 gms, sugar dissolved in 20 gms, eats gave ei 0°118°C. 
= 366. 
M.W, of lactose C,,H.0), + HO. = 360. 
have obtained the followin c 
several samples of butter-fat. Roam vl pet spcmeeoe 
tained from the village of i popes personally superintending t the 
: Fiera 'e 
a and iia , 1906, from animals with calves of differ- 
= eubeateapaaeaes cee eee tna PONDS eee 
* . 
that ane ates Ma Mr. Dutt, Profescor of Agriculture, Shibpur Colleges 
sonlelsainicaa well-marked breeds of Indian buffalo, and that the names 
given merely refer to the localities in which the animals live. 
