88 DR. J. W. JENKINSON ON THE [Feb. 6, 



bath and left a brownish residue. On extraction with absokite 

 alcohol the same yellow solution with a reddish tinge is obtained 

 as before, giving the bands with exactly the same readings. 



" This alcohol solution was again evaporated down and the 

 residue dissolved in ether, which dissolved a considerable portion, 

 giving a yellowish solution with a reddish tint ; this gave two 

 bands : — 



(1) \ 593 or 594-5 - 577, 



(2) X 558 — 533-5. 



" This shifting is due to the influence of the solvent. 



"There also seems to be a slight shading between X 516 and 

 496? 



" This ether solution was evaporated down and the brown 

 residue extracted with chloroform ; this formed a reddish solution, 

 which contained oily-looking red drops floating undissolved on the 

 surface. 



" The two bands now are : — 



(1) X 596 - 574, 



(2) X 558 - 533-5. 



" The first is not as dark as the second. 



" A faint greenish fluorescence seems to be present in all these 

 solutions ; it does not disappear on filtering. 



" The chloroform solution was next evaporated down and the 

 residue dissolved in rectified spii'it. 



" The addition of ammonia or of caustic soda to this solution 

 causes precipitation, and, in the filtrate, diminishes the intensity 

 of the colour of the solution and the shading of the bands, but 

 does not appear to alter their position. On adding one drop of 

 sulphuric acid the fluid becomes slightly turbid, and the two bands 

 in the filtrate have disappeared to be replaced by two others, 

 (1) before D and (2) in the green, which are difiicult to measure, 

 but whose position is approximately as follows : — 



(1) X 607 - 596, 



(2) X 568 - 547. 



" These recall at once the bands of acid hsematoporphyrin, the 

 positions of which are : — 



(1) X 610-591, 



(2) X 585 — 567-5. 



" It is to be noted, however, that the addition of alkalis does 

 not produce the usual four-banded spectrum of alkaline heemato- 

 porphyrin, but Garrod and others have described in urine a two- 

 banded neutral heematoporphyrin spectrum. 



" Fearing that possibly ^four-handed alkaline h^ematoporphyrin 

 spectrum might have been present but have been passed over 

 owing to the weakness of the solution, a layer of fluid (absolute 



