1910.] 



MORPHOLOGY OF KIMERIA AVIUM. (iOl 



Plate LVII. 



Fig. 37. Micrognmetocyte or male niothei- cell. 



38-40. Nuclear fragmentation of microfi'ametoc.vtc. In fig. 38 remains of 



parent karyo.sonie seen. In tig. 40 young niicroganietes arranged 



along meridians. 

 41, 42. Young microgametes at periphery of mother cell. 

 43-45. Se])aration of the clusters of microgametes. 



46. Fri'C inirrogametes. 



47. Fertilisation. One microgamete has gained access to the female pronuc-lciis. 



The micropyle has closed, and the microgametes left outside will 

 degenerate. 



48. Fertilised oocyst. Zygote nucleus beginning to form a fertilisation spindle. 



Remains of microgametes on outside of cyst. 

 40. Zygote in oocyst just after fertilisation. 

 50, 51. Show " fertilisation spindle " — not very clearly differentiated in Eimeria 



avium. 



52, 53. Longitudinal and transverse sections of oocysts, containing rather a 



large amount of reserve material — as judged hy staining (lueniatoxylin). 

 In tig. 53 the zygote nucleus shows signs of dividing. 



54. Oocyst with four nuclei, one ready for each s])orohlast. Safranin, Lichtgrvin. 



55. Small oocj'st with four round sporohlasts. Paracarmine. 



66. Oocyst, showing .segmentation into four sporohlasts. From lumen of cjccum 



of dying grouse chick. 



57. Oocyst, Avith four ovoid sporocysts, and cystal residuum, containing minute 



dot of chromatin (? remains of karyosome of macrogamete). Cut 

 obliquely. 



58. Oocj'sts with four sporocysts showing commcnccjncnt of ditlerentiation 



(polar separation of nuclei) into sporozoites. Paracarmine. 



59. 60. Sporocysts, each with two sporozoites, Dehiscence represented in fig. 60. 



Plate LVIII. 



Mostly from fresh preparations. X 1000. 



Fig. Gl. Zygote nearly filling oocyst. Culture of caical contents of grouse containing 

 such oocysts made and examined for four days subsequently. 

 62, 63, 64, 66. Development at beginning of second day. 

 65. Cyst uninucleate, such as is frequently seen in fresh faces. 



67. Oocyst with four sporohlasts, as seen at end of second day, and on third day. 



68. Oocyst with four ovoid sporohlasts, as seen in cultures on tlie third day. 

 68-70. Illustrate three common forms of oocyst of E. aviiim — oval, egg-shaped, 



and rounded. There is variation among the oocysrs. 



71. Oocyst with four sporocysts, in each of which sporozoites are differentiating. 



72. Oocyst with four sporocysts, nearly ripe, each containing two sporozoites. 



From partially dried c*cal droppings, taken from grouse moor. 



73. Ripe oocyst dehiscing — one sporocyst outside, with sporozoite nuclei at 



poles, and central vacuole. Sporocyst inside oocyst shows polar 

 vacuoles. 



74. Free sporocyst, from fauces. Sporocyst contains two sporozoites, arranged 



'■ tete beche," and sporal residuum. 



75. 76. Sporocysts, each with two sporozoites. 



7". Oocyst, commonly occurring in fresh droppings. 



78. Oocyst with somewhat square ends. 



79. Oocyst with depression (thin place in cyst-wall) at one end. 



**'-'■ ^ygotf i" oocyst — zygote nucleus dividing into two. Stained preparation. 



81. Oocyst containing two precociously separated sporohlasts. There should 



he, noimally, four sporohlasts. I'rohably the oocyst represented will 

 degenerate. Stained pieparation. 



82. Oocyst with two sporohlasts. X'ery few of these seen. Possibly abnormal. 



Tlij four sporohlasts should be formed almost concurrently in the 

 normal cyst. 



53. Oocyst showing protoplasmic contents in form of cone-like projection 



attached at one point to the cyst. Development retarded, after 

 kee])ing three wc(>ks in watev. 



