096 DIt. 11. B. KAXI'IJAM OX TIIIC [iMmV 3. 



eitiiertlio pnrasite iiniler<f()es!i iiiu]ti[)li(';i( ive stnge in souk! iiiteniMl 

 orj^aii (tliough it is stated tliat the intei-nal organs of tlie fowls 

 wei-e exaniineil), or a. latent phase witliout increase in rnnnliers of 

 the parasite occurs, oi- else a re-infection of the host takes place. 

 I luive found schizogony of L. lovati (see next section) in the sj)leen 

 of the host, but so far neither time nor material have been 

 available for my observing any possible periodicity in the gameto- 

 cytes of L. lovati, though such may occui-. 



Gametocytes were found to be rare in ihc p( riplu'ial lilooil, 

 moi-e numerous in heart-blood, and were also seen in snirars ot 

 the liver and spleen of infected grouse. 



( d ) fSch i::o(j() iii/. 



Schizogony in the avian Leucoeytozoa has not been recorded 

 before. However, in my investigations of L. lorati, 1 ha\e 

 succeeded in finding schizonts of this Leucocytozoun in the spleen 

 of two infected birds (PI. LX. figs. 23-28). i saw living 

 Leucoeytozoa, in the heart-blood of these birds, and innnediately 

 made fresh smears of the internal organs. Lii the spleen 

 were found rounded or ovoid bodies — the scliizonts — with thin 

 walls, coloured red with Giemsa's stain. Tliese schizonts 

 contained many inerozoites (figs. 25, 26). The capsides of the 

 schizonts are i)robal)ly formed, at least in part, by the remains of 

 the host-cell (fig. 26). The protoplasm of the schizont appeared to 

 be slightly granulai- (fig. 26). 8ome of the schizonts already con- 

 tained a few nuclei in process of multi])lication by amitotic binary 

 fissions (figs. 28, 24), while other schizonts contained small vei-- 

 micular merozoites (figs. 25, 26) which ultimately escape from the 

 mother cell (tig. 2(5) and may be found free in spleen smears 

 (figs. 27, 28), when their mode of origin and general appearance 

 are (-learly grasped. A small amount of residual [)rotoplasm is 

 left behind in the thin mend)ranous remains of the schizont 

 which has just shed its merozoites. The schizonts are from 1 1 /( 

 to 14 ju by 8 ju to 11 ji(, and the merozoites are about 7 /( in length. 



The birds whose spleens contained schizonts of X. lorati were 

 not infected with any other Hipnioprotozotin. 



Preparations made from the bone-marrow of infected birds. 

 in which schizogony might also take place, did not, exhibit 

 developmental forms. 



(e) CoiicliidiiKj Rciiiarls. 



The method whereby LeAicocjftozooti, lorati is transferred from 

 grouse to grou.se has not yet been shown with certainty. Tlie 

 vector oi- second liost of />. lorati will probably be found in some 

 l>lood-sucking in.sect, uliieli ingests the parasites from one 

 grou.se and transfers them to the next bird from which it ol)tains 

 l)loi)d ; or |ieili.ips there is a cycle of development of the parasite 

 inside the .\rtlu'opod vectoi-. 



The gi'ouso-rty (Onilt/innii/ia lai/nj)nr/i\) suggests itself a> a 



