700 Dlt. II. It. KANTHA.M UX TlIK [AfilV .'!. 



<'re(Miin of grouse is best uiimed TricJioniunas ebartlii, thoiigli 

 l*]))eitirs original tigures published iu 1862 do not especially 

 suggest a typical Trichouumas. However, the somewhat peculiar 

 shapes figured by Eberth are perhaps ilue to the fact that the 

 organism is very easily deformed. 



In grouse both typical Y'richomoiuis (PI. LXI. figs. 43 4(j) and 

 Monocercomoiias (fig. 48) forms were seen in life as well as in 

 fixed and stained prepaiutions (fixed wet with osmic acid and 

 stained with DelafiehUs ]i{euiatoxy]in or Giemsa's stain). The 

 bodies of the parasites vary from 8/x, to 13yu by 6 ^ to i) f.i. 



TrlcJionionas ebertiti possesses three tiagella disposed around a 

 sligiit cytostomic depression (Hgs. 48-45), and arising inside 

 the body close to a, chromatin granule, the hlepharuplast or 

 kinetonueleus. The tiagella are often much entangh'(l. Tliti'e is 

 an undulating membrane with a, flagellar Ijorder and short free 

 flagellum ; the membrane possesses also a chromatic base line on 

 the body as seen in some specimens (fig. 45). There is also a 

 skeletal organella arising at or close to the blepharoplast and 

 running backwards to the posterior (non-flagelku-) end of the 

 l)ody, where it may project a little (figs. 43-45). This stiff 

 skeletal rod — which stains blue with (Jiemsa's stain — is the 

 a\ostyle. A row of granules or blocks may be seen alongside the 

 axostyle and base line of the membrane — better marked in some 

 specimens than others (figs. 43-45). The nucleus is situated 

 lather neai-er tlie flagellar end of the body close beneath the ble- 

 [iharopkist, and is fairly large and more or less spherical. 

 C'liromatin granules are seen within it during the resting state, 

 and a nuclear membrane is present (figs. 43-46). 



7'richoinoiias eherthi divides longitudinally. Although 1 have 

 carefully searched for dividing forms, I have only seen tliem on two 

 occasions, and then in stained preparations. One of the dividing 

 forms is shown in fig. 49, but I had not sufficient material to 

 follow the details of division. The process of division is well 

 described by Dol)ell (1909) for Trichoiiionas hairachorinii, and l)y 

 Wenyon (1907) for T. intesi'malis in the mouse. 



Encvstment* of 2\ eherthi occurs (fig. 50). The organism 

 giadually becomes oval in outlint-, and its body substance con- 

 i-entrates after gradual absorption of the flagella, undulating 

 iiKMiiliiano and axostyle (fig. 50). A thin gelatinous cyst is seen 

 in I hi- specimen drawn (fig. 50). Round, contracted, unencysted 

 i'oiins were also seen ; these are probably resting f'oi ms. 



A tlagellate, irregular, non- motile forms with nucleus and .axostyle 

 are occasionally seen. Aum-boid forms are, I believe, the result 

 (,f degeneration. 



Sometimes, in stained smeai's of the ca'cal contents of grouse 

 containing Trichomonas, I have seen curious ovoid or tigure-of-8 

 holies which suggest much deformed specimens of 7'rich(>i)iatias in 

 which the axostyle acts as a rod around which the flagellar border 



* li. I'iuisi hrt'* rcceiith' iiiitoil riii-xs(niiMit in 'rriclioiuDiiiiils (.Vrcli. f. I'mtisti'ii- 

 kuiidc, \ix. p. •_>;{'-'). 



