7()'2 DIt. II. It. lANTll.VM OX THE [iNImV 3, 



occur iilong tlic l)()ily (ligs. ;")], r)2, 55). The incinhraiK; with its 

 clirouijitic* border i.s often clo.sely (H)iitrncte(l agaiii.st the body 

 (tigs. 53, 55). The chromatin of the Spirochjete i.s nsually 

 pre.sent in the form of a number of Ijaivs (tigs. 51-57) whicli ai-e 

 probably dispo.sed on a helix jis is typicil for iSpirochaetes. 



After examining many specimens of the pai-asite it was seen that 

 aS'. luvati exhibits nioi-pliological variation. Thus, the ends of 

 tlie boily usually tapci- (ligs. 58-57). but occasionallj' are some- 

 wliiit rounde I (tigs. 51, 52). Also, the H[)irochietes ai-e of diU'ereiit 

 leuirths and bi-eadths, the results of growth and division. 



]\[ulti|)licatit)n of S. lovati by both longitudinal and transverse 

 division has i^een seen in life. Division resend)les that of S. re- 

 currentis and ;S'. dattom, where a periodicity in the dii'cction of 

 division occurs (Fantham and Porter, 1909). Broad 8pirochiietes 

 (tig. 51) have been seen to divide longitudii:ial]y (fig. 5(5). Longer 

 Spiroch;etes (fig. 54) often divide transversely (fig. 57), elongating 

 somewhat as they do so. 



Tiie modes of multiplication and the processes of growth are of 

 the utmost importance in elucidating the morphological viiriation 

 of .Spirocha^tes, 



(c) Rhizopoda. 



Am(i:i;a (Exi^lucl'ha) lagopodis, sp. n. 

 (Plate LXI. figs. 58-65.) 



Living Amcebfe were seen by Dr. Shipley (1908-9) in fresh 

 faeces of grouse. I have, on a few occasions, observed Anuebie 

 in the recently voided faeces and in the rectum and intestine of 

 grouse. The living organism was examined both with ami without 

 staining intra vitam, and a few stained pr-eparations wei'e made 

 with Delafield's lijematoxylin after fixation with corrosive-acetic- 

 alcohol or osmic vapour, but the whole of the material was very 

 scanty. 



In structure tlie Anuvlia exhibits ectoplasm and emloplasia 

 fairly well differentiated (PI. LXL tigs. 58-63). The Amceba 

 moved slowly l)y sending out pseudopodia in the manner usual to 

 these organisms, the pseudopodia being tii-st chietiy composed of 

 ectoplasm. Very few pseudo})odia wei'e sent out at any one time. 

 There is a nearly central nucleus, often possessing a karyo-some. 

 Food vjicuoles containing; partially digested food particles and 

 Viacteria were seen (tig. 61). The granular endoplasm may i-ontain 

 a vacuole (figs. 58. 59). 



Amdeboid organisms, from tlie incgularity of their sha})e. are 

 not easy to measure ])recisely. More or less roundi'd forms may 

 measure (W /.i in diameter (Hg. 61 ) with a inicleus !()/< in diameter. 

 Other specimens of the pai'asite (figs. 59, (>(), (i3) measure fi'om 

 *J() n to AO ft across, with a nucleus of 5^/ to 8/1 in diameter. 



In prepanitious stained with Delatiehl's luematoxylin the 

 nucleus was .seen to be spherical (Hgs. 58-61) with a nticlear 

 menil>rane on which the chromatin coulil soiiietiiues b(> clearlv 



