22 PROF. E. A, MINCHIN ON PROTOZOAN [Jan. 12, 
most commonly found, one in which there are two karyosomes, 
either subequal, or markedly unequal, in size (figs. 85, 86, 93). 
But in many of the large forms the karyosome may be further 
broken up into three or even four parts, usually differing greatly 
in size (figs. 89-92). Thus, with endless variations in detail, a 
similar type of nuclear structure occurs with monotonous regu- 
larity in all the preparations stained with iron-hematoxylin ; 
always an oval space containing one or more deeply staimed karyo- 
somes. What may be the significance of the disruption of the 
karyosome I am unable to say. 
The kinetonucleus appears after iron-hematoxylin as a rounded 
or rod-shaped body of minute size. After staining by the Roma- 
nowsky method it appears very much larger and of various shapes 
which are seen in my figures. Near it a distinct blepharoplast 
can be made out, from which the flagellum arises directly. The 
free flagellum is usually of considerable length. The undulating 
membrane does not stand out so boldly from the body as in some 
species of fish-trypanosomes. In one preparation I saw distinctly 
a line under the flagellum (fig. 82), indicating doubtless the limit 
of the extension of the endoplasm into the undulating membrane. 
Only in one specimen was I able to make out myonemes (fig. 84). 
They appeared as delicate lines, about three being visible on the 
side of the body, a number which may correspond to the total of 
eight alleged to be typical of trypanosomes. 
3. TRYPANOSOMA REMAKI Lav. & Mesn. (Plate II. figs. 20-26.) 
The trypanosome of the Pike has been described by Laveran and 
Mesnil (Arch. Protist. i. 1902, pp. 482-486, figs. 1-9). I have 
very little to add to their description of this parasite, which I 
found in four out of the five pike examined by me at Sutton 
Broad. Laveran and Mesnil have noted that this parasite has a 
wide distribution in Europe, its occurrence having been noted by 
several naturalists. 
Laveran and Mesnil noted two forms which they distinguished 
as var. parva and var. magna. 1 found these varieties very dis- 
tinctly marked in the pike examined, and without any transi- 
tional forms connecting the two extreme types. Both var. parva 
and var. magna are subject to slight variations in size, but I found 
no difficulty whatever in distinguishing the two types in my 
preparations, whether fixed by osmic vapour or by the ordinary 
method of drying. The var. parva (figs. 20-22, 25) is clearer, 
with but few coarse granulations, its undulating membrane is 
shallow and does not stand out sharply from the body, and its 
free flagellum is longer, relatively and absolutely, than that of 
var. magna (figs. 23, 24, 26), which contrasts with it not only in 
greater dimensions, but also in its opaque cytoplasm full of coarse 
granules staining reddish with the Giemsa stain, and in its very 
prominent undulating membrane. The kinetonucleus also seems 
to be slightly larger in var. parva than in var. magna; but I am 
not able, unfortunately, to make precise statements concerning 
