26 PROF. E. A. MINCHIN ON PROTOZOAN [Jan. 12, 
usually a single karyosome. In the ordinary forms the karyosome 
is small, often no larger than one of the coarser cytoplasmic 
granules, with which it may be confused very easily, so that at 
first sight the trypanoplasms appear to have no trophonucleus. 
Tn the only specimen of the large forms which I have seen stained 
by this method, the karyosome was very large and almost filled 
the nuclear space (fig. 65). In one specimen of the ordinary 
forms I found what appeared to be nuclear division (fig. 62); two 
karyosomes connected bya distinct curved black line, one of them 
in the ordinary position of the trophonucleus, the other further 
forwards, on a level with the kinetonucleus. In another specimen 
the karyosome appeared to be budding off a smaller part (fig. 59), 
perhaps as a preliminary to division. 
The difference between the nucleus in specimens stained by the 
Romanowsky and the iron-hematoxylin methods is very striking. 
One sample, however, of Giemsa’s stain which I used gave 
different results from all others, for some unexplained reason ; 
with this sample the flagellum stained blue instead of red, and 
the trophonucleus appeared as a small granuie (fig. 36), just as in 
the iron-hematoxylin preparations; the kinetonucleus appeared 
very large. Hence all the trypanoplasms stained with this 
sample of stain gave the impression, at first sight, of lacking a 
trophonucleus. 
The kinetonucleus appears very large after the Romanowsky 
stain, much smaller after iron-hematoxylin, but in both cases 
larger, as a rule, than the trophonucleus. There are two types 
of form exhibited by the kinetonucleus : a more rounded or oval, 
broader type (figs. 57, 59), and a more elongated, band-hke type 
(figs. 61, 64); the latter often appears to have a small piece at 
the anterior end constricted off from the main body (figs. 58, 64; 
compare Keysselitz, Arch. Protist. vil. p. 36, fig. 45). The 
kinetonucleus stains an even black in iron-hematoxylin, a deep 
purple after Giemsa, and shows no structural detail. Having 
only seen two specimens of the large form of this trypanoplasm, 
T am unable to say if distinct types of kinetonucleus are exhibited 
by it, as by the large forms of 7. keysselitzi. In front of the 
kinetonucleus are two minute blepharoplasts from which the 
flagella arise. After the Romanowsky stain the blepharoplasts 
appear a fair size and are distinctly seen ; after iron-hematoxylin, 
on the contrary, they are very minute and often appear as a single 
granule, being close together and difficult to resolve imto two 
distinct dots. When the anterior end of the body is drawn out, 
the blepharoplasts appear sometimes one behind the other, and 
with a considerable interval between them. 
The following is an attempt at a brief diagnosis of 7rypano- 
plasma gurneyorum:—Oceurs under two forms, ordinaryand large ; 
trophonucleus near middle of body, with single karyosome ; 
kinetonucleus compact or drawn out, large; anterior flagellum of 
moderate length ; free portion of posterior flagellum very short. 
Host, Hsox lucius, Norfolk. 
