30 PROTOZOAN BLOOD-PARASITES OF FRESHWATER FISHES. [Jan. 12, 
Fie. 15. Encysted form of Trypanosoma perce, from the same slide as 9 and 10. 
Osmie vapour, absolute alcohol, Giemsa. 
Fig. 16. Young form of 7. perce? or vermicule of a hemogregarine? From 
the same slide as the last. 
Figs. 17-19. Leucocytes of the Perch, showing peculiar enclosures. From a 
preparation dried off, fixed with absolute alcohol, and stained with 
Giemsa’s stain. 
eNom) JUG 
Figs. 20-26. Trypanosoma remaki of the Pike. 20-22 & 26, small forms (var. 
parva) ; 28, 24, & 26, large forms (var. magna) ; 20-24, from pre- 
parations fixed with osmic vapour followed by absolute alcohol; 25, 26, 
from preparations dried off, fixed with absolute alcohol, all stained 
with Giemsa’s stain. 
Figs. 27-29. Trypanosoma tince, from the Tench. 27, dried, absolute alcohol, 
Giemsa ; 28, 29, osmic vapour, absolute alcohol, Giemsa. 
Figs. 30-32. Trypanosoma abramis, from the Bream. 30, 31, osmic vapour, 
absolute alcohol, Giemsa; 32, dried off, absolute alcohol, Giemsa. 
Figs. 33-39. Trypanoplasma gurneyorum, from the Pike. 83-36, osmic vapour, 
absolute alcohol, Giemsa; 37-39, dried off, absolute alcohol, Giemsa. 
Prave IIT. 
Figs. 40-44. Trypanoplasma keysselitzi, from the Tench. 40, 41, large forms ; 42—44., 
ordinary forms; 40-43, osmic vapour, absolute alcohol, Giemsa ; 
44, dried off, absolute alcohol, Giemsa. 
Figs. 45-48. Large uninucleate leucocytes of the Tench, showing peculiar pink- 
staining enclosures; preparation dried off, fixed with absolute alcohol, 
stained with Giemsa’s stain. 
Figs. 49-54. Trypanoplasma abramidis, from the Bream, 49, fixed with sublimate- 
acetic (95:5); 50-52, fixed with osmic vapour followed by absolute 
alcohol; 53, 54, dried off, fixed with absolute alcohol; all stained 
with Giemsa’s stain. 
Fig. 55. Trypanoplasma borreli, from the Rudd. Osmie vapour, absolute 
alcohol, Giemsa. 
PrarE TV. 
Figs. 56-65. Trypanoplasma gurneyorum, trom the Pike. 56, large form, from a pre- 
paration stained with Giemsa’s stain, all the others from preparations 
stained with iron-hematoxylin; 56, osmic vapour, absolute alcohol ;. 
57-61 & 63, sublimate-acetic (95:5); 62, 64, 65, Mamnn’s picro- 
corrosive with formol. 
Figs. 66-73. Trypanoplasma keysselitzi, from the Tench; all from preparations 
stained with iron-hematoxylin. 66-69, large forms; 70-73, ordinary 
forms; 66, Schaudinn’s fluid; 67 & 70, sublimate-acetic (95:5) . 
68, 69, & 71-73, Mann’s picro-corrosive with formol. 
Figs. 74-76. Trypanosoma tince, from the Tench, all from preparations stained 
with iron-hematoxylin ; 74, Schaudinn’s fluid direct ; 75-76, Schau- 
dinn’s fluid preceded by exposure to osmic vapour. 
Fig. 77. Trypanoplasma abramidis, trom the Bream, sketched living. 
Prare V. 
Figs. 78-93. Trypanosoma granulosum, trom the Kel, all from preparations stained 
with iron-hematoxylin. 78-82 & 85-93, fixed with Mann’s picro- 
corrosive-formol mixture; 83, 84, fixed with sublimate-acetic (95 : 5). 
78-80. Small forms. 
81-82. Medium-sized forms; in 82 only the posterior part figured, to 
show the double line in the undulating membrane, the outer line 
representing the flagellum, the inner line representing the limit of the 
extension of the endoplasm imto the membrane. 
83. Large form, anterior half, the stain but slightly extracted, showing 
the cytoplasmic granules ; whole length of the flagellum not drawn. 
84. Large forms, anterior half, stain under-extracted, showing,\ the 
myonemes ; whole length of the flagellum not drawn. 
85. Large form. 
86-93. T'rophonuclei of different specimens, all from the same prepar- 
ation, and all from large forms. 
