88 DR. A. E. BROWN ON THE [Jan. 12, 
It cannot be said that the results reached with lemurs are 
equally exact. Indeed, at the present time, we are not disposed 
to rely much upon the charts in this group, except in cases of 
positively bad reaction. The greater frequency of doubtful cases 
here, than with monkeys, is primarily due to the greater amount. 
of normal individual irregularity, especially in regard to the night 
drop. ‘This is true even of diurnal species, as the genus Lemur, 
in which it 1s sometimes well marked, at others absent. ‘To. 
illustrate this two post-injection curves are given in Chart D: 
(text-fig. 4, p. 87), of a female Lemur varius, on the first of which, 
taken in April 1907, had she been a monkey she would have been 
unhesitatingly condemned. In July she gave a good chart, 
differing from monkeys only in somewhat advancing the hour of 
the night minimum, She died September 8th, without any trace. 
of tuberculosis. 
There is some reason to believe that on the whole lemurs are: 
less susceptible to infection through the usual channels than 
monkeys, though by no means immune. 
At the present time, after three and a half years of systematic 
observation, the findin gs we believe to be justified by the fact that 
more than a year has passed without evidence of the existence of 
tuberculosis in the Monkey House, are these :— 
1. In every case where a positive reaction has followed injec- 
tion we have found tuberculosis on autopsy. 
2. In negative charts, where no rise of temperature appears, 
nor perturbation of the daily rhythm, we have had no reason to. 
believe that the disease exists. It has been absent in every such 
case after a forty-eight hour observation, where animals have 
subsequently come to autopsy. 
3. In intermediate charts, neither positive nor negative, safety 
lies in nicety of judgment on the part of the observer, this being 
mainly a matter of experience. But a very small percentage of 
such cases have been tuberculous. 
4. In very advanced cases of general tuberculosis the usual re- 
action does not always follow injection, but we have found such 
cases to be indicated bya notable drop in temperature, and proved 
by early death. 
The difficulties in the way of applying the temperature reaction 
test generally throughout a zoological collection are obvious. The 
effort, risk, and various uncertainties involved in the frequent 
handling of large and powerful ungulates or carnivores, to 
procure the indispensable data, are so great that it is probably 
impossible. 
Tn this aspect the eye test of Calmette, and skin tests, such as 
that of Ligniéres, seemed to be of some promise; but it is un- 
fortunately true that experiments made by the present pathologist, 
Herbert Fox, M.D., with these tests upon monkeys known to be 
tuberculous, have not fulfilled expectations. 
We are naturally led to the general subject of mammalian 
