326 DR T. GOODET ON 



I made one or two film preparations, which, after fixation and 

 staining, showed the presence of large numbers of amoebte, 

 together with the fla.gellate Gercomonas longicauda. In going 

 carefully over one of these preparations, I discovered a number 

 of interesting stages of dividing nuclei ; and though I have not 

 obtained a very complete series of these, I have made out 

 sufiicient to show that I am dealing with an organism hitherto 

 mid escribed. 



(a) Structure. 



There is nothing remai-kable in the appearance of the ordinary 

 individuals. The body presents an endless variety of shapes, 

 and the pseudopodia are very irregular and lobose, whilst the 

 distinction between ectoplasm and endoplasm is not at all clear. 

 So far as 1 can ascertain, the endoplasm is not alveolate in 

 structure. At any rate, if alveoli are present they are not large 

 and distinct like those in Amoeba lawesiana and Amoeba glebce, 

 for in only one of the animals could I make out anything at all 

 approaching alveoli. I do not wish to emphasize this point, how- 

 ever, for the preparation was slightly over-difl'erentiated and the 

 cytoplasm in all the amoebte was only very faintly stained. On 

 the whole, the endoplasm appeal's very finely gianular in 

 structure, with somewhat denser masses scattered about in it ; 

 and the figures which illustrate this account represent it fairly 

 accurately. 



The resting nucleus consists of a central deeply staining 

 kaxyosome, separated by a clear zone from the nuclear mem- 

 brane, with which it appeal's to be connected by very feebly 

 staining strands stretching across the zone at various points. 

 I have not been able to make out the presence of a ring of 

 granules just within the nuclear membrane as in Amoeba 

 laivesiana. The pi'incipal measurements are as follows : — 



Length of body 12-15^, though this measurement is not of 

 much value because of the very irregular shape of the 

 organism. 

 Diameter of nucleus, 2-75--3^. 

 Diameter of karyosome, 1*8-2 jjl. 



It is thus rather smaller than Amoeba laioesiana. 



(b) Iieproductio7i. 



The body does not become globular during nuclear division 

 as in Amoeba glebai and Amoeba lawesiana, but retains its very 

 ii-regular appearance. The karyosome in the earliest stages of 

 division loses its rounded sh.'xpe and increases in size. At the 

 same time it begins to break up into a number of ill-defined 

 granules, which appear to rest on a matrix which stains only 

 feebly. I have only encountered a few of these early stages, and 

 therefore cannot give much information concerning the changes 

 which go on at this period. 

 ' The final result of the break-up of the kai-yosome is the 



