130 OBSERVATIONS ON THE CALIFORNIA VINE DISEASE 
The facts that I have just related in regard to the behavior 
of the chloroplasts refer almost exclusively to the cells of the 
palisade tissue. In the cells of the lacunose tissue the chloro- 
plasts become vacuolate, but remain small, as a rule, and their 
resorption progresses gradually. Тһе occluding of the lumen of 
these cells does not occur so rapidly and is rarely so dense or dark 
in color, even in the most rapid cases of death, as that of the 
palisade cells. 
I remarked, in a previous passage, that the row of cells of the 
lacunose tissue abutting on the palisade layer was very free, when 
compared to the other cells of the same tissue, from deposit. 
When the lumen of these cells is free from deposit the chloro- 
plasts not only become vacuolate but fragment and, it would 
appear, decompose with the formation of oil-like bodies, which, 
when small, stain like the chloroplasts, but do not color, when 
larger, as vividly, if at all, in acid fuchsin, which fact leads me to 
believe that, if originally largely decomposing chloroplastid 
remnants, they grow by accretion of other proteid substances; 
this is brought out clearly when sections are stained with rosani- 
line: the smaller bodies will appear red, the others violet. They 
all stain, however, more vividly in safranin and eosin than the 
chloroplasts themselves, which would tend to show that their 
composition is fairly complex. | 
During the course of my remarks on the behavior of the 
chloroplasts- I have frequently made mention of the homogeneous — | 
substance filling the cell lumen. The various stages of chloro- 
plast degeneration we found to depend on the relative amount and 
rapidity of production of this substance. It is therefore, im- 
portant for us to determine the nature and origin of the homo- | 
geneous deposit and its homologues, the globules and granulat , 
matter. This I will now attempt to do. e 
From my observations on the degeneration of the chloroplasts 
it plainly appears that the substance occluding the cell lumen is 
not a product of their decomposition. That from the decomposi- 
tion of the chloroplasts there appears to result, in some cases, the 
formation of oil-like bodies is no contradiction to this statement 
The latter form of decomposition is rare. Furthermore, the n 
