LEVINE: Two SPECIES OF POISONOUS MUSHROOMS 181 
The plants used in making this infusion are shown in PLATE 6, 
FIGURES 1-6. 
Infusion No. 3 was made by grinding 13 gm. of Panaeolus 
venenosus to which sufficient water was added to make a filtrate 
of 50 c.c. | 
Infusion No. 4 was made from 25 gm. of Agaricus campestris 
treated as described for infusion No. 3. This infusion served as 
a control. 
No. 5 was an extract made by grinding 5 gm. of P. venenosus 
which had been dried at room temperature and 45 c.c. of distilled 
water. The filtrate was evaporated at 25? C. giving .5 gm. of a 
brownish-yellow mass. This was mixed with 10 c.c. of distilled 
water. 
Extract No. 6 was made by rubbing up the residue on the filter 
obtained in making extract No. 5, with absolute alcohol and filter- 
ing. The filtrate was evaporated and the mass was shaken up with 
25 c.c. of distilled water. 
Infusion No. 7 was made like extract No. 5, but with the excep- 
tion that the filtrate was not evaporated. There were 30 c.c. of 
the filtrate, which was greenish yellow at first and gradually be- 
came a darker greenish yellow. 
Infusion No. 8 was made by thoroughly crushing ro gm. of 
fresh P. venenosus to which was added 40 c.c. of distilled water. 
This was allowed to stand for 24 hours before filtering. 
Infusion No. 9 was made by rubbing 25 gm. of mature P. 
venenosus (PL. 7, FIGS. 9-15) with 25 c.c. of distilled water. The 
pulpy mass was allowed to stand for 24 hours before filtering. 
The filtrate was no further diluted but sufficient sodium chloride 
was added to make the solution isotonic with the muscle tissue of 
the turtle. 
Infusion No. 10 was made of Panaeolus retirugis (EL. 7, FIGS. 
16-19); 12 gm. of plants of this species were macerated with an 
equal quantity of distilled water. Sufficient sodium chloride was 
added to make the solution isotonic with turtle muscle tissue. 
The mass was filtered 24 hours after maceration. The filtrate 
was black in color and had the odor of crushed lawn grass (Poa 
Pratensis). 
Infusion No. 11 was made of a species of mushroom which re- 
