182 SEMI-CENTENNIAL OF TORREY BOTANICAL CLUB 
sembled in all respects P. venenosus with the exception that it had 
a comparatively short stipe and no marked tomentum at the base 
of the stipe. This plant I shall describe later as a form of EF. 
venenosus. To 15 gm. of these plants was added 15 c.c. of dis- 
tilled water. The filtrate was diluted to make 20 c.c. of fluid and 
sufficient sodium chloride was added to make it isotonic with the 
muscle tissue of the turtle. 
Infusion No. 12 was made of mature specimens of P. venenosus. 
20 gm. of fresh mushrooms were macerated with 30 c.c. of .7 per 
cent saline solution. The liquor was permitted to stand for 
twenty-four hours before filtering. 
Infusion No. 13 was made of 10 gm. of P. venenosus macerated 
with то c.c. of .8 per cent saline solution three days after the plants 
were collected. The infusion was filtered twenty-four hours later. 
The effects of such aqueous infusions of Panaeolus venenosus 
on guinea pigs and rabbits were tested, first, by intraperitoneal 
injections; second, by subcutaneous injections; and third, by 
feeding the infusions or the fresh mushroom mixed with small 
pieces of lettuce to the animals. In making the injections aseptic 
methods were followed. 
THE EFFECT OF INTRAPERITONEAL INJECTIONS ON GUINEA PIGS 
AND RABBITS 
The results of the intraperitoneal injections are given in 
TABLE I. The quantity of infusion injected varied from 15 to 
2 per cent of the body weight of the animal. In some experiments 
(see table) the infusion was used as made. In other cases I c.c. 
of the infusion was mixed with 4 c.c. to 9 c.c. of physiological salt 
solution, distilled water or tap water. The results obtained by 
injecting the given quantities of Panaeolus venenosus infusion 
invariably caused the death of the guinea pig in about twenty-four 
hours. After the injection was made the animal usually was 
restless and moved about in an apparently dazed condition. This 
was followed by a period of lethargy, from which he never 
recovered. During this period he generally assumed a char- 
acteristic position. The head was retracted between the shoulders 
and the eyes were closed. When the eyes were open the 
animal usually appeared stupid and dazed. Noises made by 
