STUDIES IN PLANT REGENERATION 203 
after 58 days all appeared on the basal surface — again the one. 
under the sand. 
Experiment то. — Longitudinal sections of parsnips, laid in а 
horizontal position in the frame, developed shoots in the neighbor- 
hood of the cambium along both sides (Fig. 3). These pieces, 
therefore, resembled Scorzonera hispanica, as described by Goebel, * 
in forming shoots along the longitudinal edges. When placed 
vertically, the shoots originated only from the apical regions, as 
was seen to be the usual procedure in the transverse pieces. 
Experiments гг aud 12.—The separate regions of the root 
were next isolated, as far as possible, in order to determine their 
capacity for independent regeneration. Тһе rind alone, when used 
as a cutting, formed a callus on the lower surface towards the 
inside, from which shoots arose after 64 days (Fig. 4). Roots 
ааста 
ОЕ 
ii GU КҮҮ 
YN dd ag 
+ 
6; 7% 
Fic. 4. Rind portion of Pastinaca sativa from the inside of which shoots have 
arisen. 
grew from the outside as in the uninjured parsnips. When the 
central cylinder alone, also exclusive of the cambium, was planted, 
a callus again appeared at the apical end. From this in four 
weeks’ time, several roots grew. No roots developed elsewhere 
in this piece, nor were shoots formed. Each region, therefore, is 
* Goebel, К. Uber Regeneration im Pflanzenreich. Biol. Centralb. 22: 492. 
