WCT Multi-state Assessment 



February 10.2003 



Part 2 — Current Distribution, Genetic Status and Densities 



Lower and upper bounds of all stream segments presently occupied by naturally self-sustaining populations of 

 WCT will be located and data quality associated with the locations of these boundaries of current distribution 

 will be rated (DQI; Tables 1 and 2). Genetic information and status will be identified for each WCT mapping 

 unit (Tables 7 and 8) along with their DQI. For Table 7, base the category detennination on information from 

 the largest sample and/or the most recent sample. Only naturally occurring, self-sustaining populations (i.e. no 

 routine augmentation with hatchery fish) of WCT will be addresses in this status review. Relative density 

 (based on a qualitative estimation of projected numbers of adults and sub-adults, excludes YOY and yearlings, 

 relativ e to site potential) for each WCT mapping segment will also be identified (Table 9). 



Table 7. Look-up table for genetic status of a population mapping segment. 



Genetic Status 



Genetically unaltered (>99.0%) as a result of introduced species interaction- tested 

 via electrophoresis or DNA 



Introgressed (hybridized) with introduced species - tested and found to be 90% to 

 99% WCT genetic material in individual fish throughout population 



Introgressed (hybridized) with introduced species - tested and found to be 75% to 

 89% WCT genetic material in individual fish throughout the population 



Introgressed (hybridized) with introduced species- tested and found to be less than 

 75% WCT genetic material in individual fish throughout population 



Suspected unaltered with no record of stocking or contaminating species present 



Potentially hybridized with records of introduced hybridizing species being stocked 

 or occurring in stream 



Hybridized and Pure populations co-exist (sympatric) in stream (use only if 

 reproductive isolation is suspected and/or testing has been completed) 



Table 8. Specify the specific information associated with genetic sampling and analysis. More than one entry 

 can be made for a population mapping segment. 



SAMPLE NO 



COLL DATE COLL ID NO FISH ANAL DATE ANAL T\TE 



% 

 WCT 



Genetic Analysis Type 



Allozymes 



PINES 



Microsatellites 

 DNA 



Page - 58 



