450 mr. h. sandon on pkotozoa feom the 
Methods. 
Cultures were made from all the samples on plates oi: nutrient agar, in 
sterile tap-water, and dilute hay infusion, and were examined at intervals for 
three or four weeks. For detecting rhizopod tests, soil or moss was taken 
from the tap-water cultures after a few days, spread out on a glass slide 
and examined with a low power, the mosses being first teased apart with 
a needle. 
Ebsults. 
1. Mud. 
These samples gave very disappointing results, possibly owing to excessive 
heat having been employed during drying. Heteromita glohosa was found 
in all of them, and Sainouron mikroteron (n. g., n. sp.) (see Appendix) in the 
mud from pond 5, the least saline of the three. Since the members of the ex- 
pedition recorded the presence of peridinians in some of these ponds, cultures 
in Miquel's solution (1) were made at the kind suggestion of Dr. E. J. Allen 
in the hopes of obtaining growths of these organisms, but, again, only the 
ubiquitous Heteromita glohosa and some diatoms were found. 
In his notes made on the spot Mr. Elton records that Uroglena volvox was 
very abundant in all the ponds in this district, and that there were also found 
flao'ellates belonging to tiie genera Euglena and Glenodinium, and eiliates 
probably belonging to the genera Vorticella, Carchesium, Dileptiis, Prorodon, 
and Colpoda. He makes the further interesting observation that whereas in 
the Bruce City ponds, which were fed with drainage water from limestone 
hills, Uroglena was very abundant, at Cape Bohemian, where the pools were in 
sandstone, this species was absent, and its place was taken by large numbers 
of Synura xivella and of peridinians. From the latter region his records also 
include Glenodinium sp., Euglena sp., and three eiliates, probably Spatlddium, 
Nassula, and OjyJirgdium. 
2. Soils. 
As will be seen from the accompanying table (pp. 456-457), the 1921 soil 
samples form an interesting series. Apart from rhizopod shells, soil 1 
vielded only two species, and in fact three out of the six cultures made from 
this sample were completely sterile. The number of species recorded from 
sample 5, on the other hand, is quite comparable to that which would be 
found in any British farm or garden soil, while the other samples fall between 
these two extremes. Soil 1, however, contained great numbers of empty 
rhizopod tests belonging to six different species, whereas in the other samples 
these organisms were much less abundant. 
An attempt was made to connect these striking differences with other 
characteristics of the soils. The bacterial numbers, counted as they were 
