582 
MR. A. M. ALTSON ON THE 
The only secondary sexual characters observed were a difference between 
the ventral pygidial plates (VIII sternite) and between the apical margins oE 
sternites VII (the 5th visible sternite of systematists) (text-fig. 1). Beyond 
these characters, unless the "ovipositor" or the asdeagus is extruded, or a 
pair are found in coition, it is impossible to distinguish the sexes externally, 
size being no guide. 
Text-fig. 1. 
Secondary sexual oliaracters of L. brunneus. Male and female ventral pygidial plates. 
ots, base of outer sheatli of ovipositor ; VII, 7tli sternite ; Ip, 7tli pleurite ; VIII, 
8tli sternite. Camera lucida, x 256. 
In the description of the male genital tube the nomenclature of Sharp and 
Muir (10) is followed, but in the case of the female reference was made to 
Packard (8), Berlese (2), Henneguy (3), and Stein (11) for terms applied to 
parts homologous to those found in L. brunneus. 
Technique and Methods. 
The technique adopted to prepare the specimens for sectioning was similar 
to that employed by Awati (1). This method gave excellent results. 
Fixation in Carnoy II for 24 hours ; three to four hours after immersion 
in the fixative the head and most of the legs and the elytra and wings were 
cut off. 
Dehydration in two washings of 90 per cent, alcohol, three hours ; two 
washings in absolute alcohol, three hours ; and one hour in a mixture of 
chloroform and absolute alcohol. 
The object was next transferred to chloroform, in which it remained for 
two days ; then into a cold saturated solution of paraffin-wax (58° C.) in 
chloroform, where it remained for two to three days. After this period the 
solution was warmed by standing it on top of the oven for 10 minutes, and 
the object was then transferred into pure molten wax (58° C.) for five to six 
hours. 
