1918.| Observations on the rust on Launza aspleniifolia DC. 249 
or near the sori. In the aecidial stage it is found in every part 
and is sed onran branched. Within the rootstock the mycelium 
is found both in the cortex as well as between cells adjacent to 
vascular ‘panies. Inside the leaves the mycelium attacks 
with varying thickness. Here and there are seen small] oval or 
spherical-fungal cells peculiarly packed together (Figs. 7 and 8). 
On the basis of a careful study of conditions, as regards 
wheat and rusts on wheat and Launea, carried on during an 
extensive tour to many re ihs in Central and Northern India, 
Doctors Cunningham and Prain thought of the possibility of 
generic relation between the rust on wheat and that on Launea. 
To prove that they carried on some inoculations on wheat with 
uredospores from Launea which resulted in infection of the 
former. Keeping in mind the chances of the spores having got 
mixed with some from diseased wheat these authors did not 
come to any definite conclusion. 
n order to settle the question of the possible connection, 
suspected by Doctors Cunningham and Prain, between the 
ecidium on Launea and Puccinia triticina (wheat rust with 
naknown aecidium) Messrs. E. J. Butler and J. M. Hayman 
UneCoa 1a 
oo ohh free germination, but not even a single plant caught 
infectio 
These authors have remarked “ that it is not without pre- 
cedent to find an aecidium and a Puccinia belonging to quite 
different fungi occupying a particular plant at the same moment. 
The aecidium on Launea might therefore belong to the Pucci- 
nia on wheat, while the Puccinia on Launea forms a distinct 
species. But direct experiment, the only true test when the 
ngs of an aecidium is in question, has failed to support 
1s vie 
On the strength of the results of the experiments referred to 
above, Dr. E. J. Butler has established the view “ that the aeci- 
dium on Launea does not belong to Puccinia triticina on 
Triticum willie. All the three spore forms on Launea found 
at be same time show undoubtedly the fungus to be aute- 
ciou 
Tnoculations on as many as thirty plants of Launea 
aspleniifolia with uredo and aecidio-spores from the same host 
were attempted by the author at Pusa in March 1916. But on 
eg of the onset of warm wea ather and poor germination of 
mene 1917, at Agra. The spores in every case showed free 
germination :— 
