ica Se eae a 
108 Journal of the Asiatic Society of Bengal. [N.S., XVIII, 
_ between the ligatures), and the toads survived for the following 
periods :— 
Toad A died after six weeks. 
Toad B was killed after eight weeks (thin but healthy 
inside and out). 
Toad C was killed after twelve weeks (health vy save for 
cysts present in the liver and mesentery). ; 
Toads D and E died after three weeks and three days. x 
Toad F died after five weeks and four days. 
Toad G died after five weeks and six days 
Toad H died after twelve weeks and four days. : 
Toad J was killed after one ear, nine weeks and two - 
days (perfectly healthy inside and out). 
In all the toads which died and in Toads B and C no signs 
of regeneration of the cut renal afferent veins were apparent 
at the post-mortem, but in the Toad J, I found that both renal 
afferent veins had been completely regenerated ( text-figure 3). 
The significance of this fact 1 shali refer to later. 
In all the toads except J, the kidneys had become appreci- 
: . Total weight of body at time of 
ably enlarged (le. the ratio oi Scone the tes tine 

pore was greater in these experimental toads than the 
corresponding average ratio determined in 56 normal toads).! 
This subject also will be discussed later. The liver did not 
~~ in size, though it obviously received more blood than 
usual. : 
The total nitrogen output in the entire amount of urine of : 
the Toad F (body-weight at time of experiment = 29'7 gms ) ue 
excreted during five consecutive days was 0:05047 gm.,? while = 
those of two normal control toads (weighing 24-5 gms. and 347 
) during the same period and under precisely the same 
conditions were 0:04125 gm. and 0°05790 gm_ respectively. . 
Thus, relative to the weight of the body, the amount of nitrogen = | 
excreted by the Toad F during this period was certainly not 2 
less than the corresponding amounts of nitrogen excreted by : 
he two control toads. The similar total nitrogen output of 
the Toads G and H ( placed together in one jar: G weighed at 
Poa ee 

io 2 aig 
ea Br sey ne ee Ee oe a a ee 

by lifting out the hind ends of the kidneys through slits made in the 
dorsal muscles. 

The g 
lowest ratio being 143-7 and the maximum 335'7. In Toads A, B, C, D,E, 
F, G, H, ratios were respectively 165-7 (and probably smaller owing 
to Tge coelomic effusion being included in the body-weight), 137°6. 
149°6, Rar (a coelomic effusion included in the body-weight). 
The whole of the urine excreted during the five days was carefully 
collected and separated from faecal matter by filtration. Total nitrogen 
determinati ‘ns by Gulick’s method. 

