118 Journal of the Asiatic Society of Bengal. [N.S., XVIII, : 
tubules can be killed (except at great venous pressure) when 
al afferent veins are perfused with 1/10,000 mercuric 
chloride. eee 
The facts supplied in the present Part II are then 
corroborative of the conclusions reached in Part I. For the 
most part they merely confirm, in the living animal], what has 
already been demonstrated by, or might be inferred from, the 
results of perfusion experiments. This confirmation however 
is, quite apart from the value of the new facts described, of 
considerable importance in view of the slur so often and 
undeservedly cast upon the results of perfusion experiments on 
the kidney. In Part III further confirmation by experiments 
on the living animal of the results obtained from the perfusion 
experiments of Part I will be supplied. 
PART IIT. 
REPETITION AND EXTENSION OF THE EXPERIMENTS OF 
GURWITSCH AND INTERPRETATION OF THE RESULTS. 
THEORETICAL CONSIDERATIONS. 
Previous Experiments. 
normal kidney, i.e. with the renal afferent vein intact. Gur- 
witsch wisely exposed the kidneys of the frogs he experiment- 
ed on from the dorsal aspect and ligatured on one side the “ reno- 
portal” vein and its confluent the dorso-lumbar (and in the 
female the small oviducal veins were cauterized with a red-hot 
needle), leaving these veins on the other side intact. Urine 
2 . . * 
or urea solution was injected into the intestine at the time 
ligatured kidney 0°8-1:0 c.c. in two hours), and, so far as 
samples of urine so obtained. 
ferring until later consideration of Gurwitsch’s state- 
ments of results obtained by him on injecting dyes into the 
and quality of urine derived respectively from kidneys 
with ligatured renal afferent veins and from normal control 


