1922.] “ Renal Portal’”’ System. 165 
Put 2nd aes tubes from 9:10-9:25 
IIL=1 c + ferric chloride =yellow coloration only. 
TIR=1 ee, (15 ™-) 
— b ecsdorprg 247 c.c. ) 
| oo. —9'25 1. oeabins Sai 1 fows, | 
POV 50 e.e. 
as ae I clamped both r.a.v., siphoned off the contents o 
o bottles and substituted i si pahie we (in 0°6% saline) 
of °K Fe(C N)s- The on Pb -started at 9°34, 
and I put 3rd pair tihen fron 9 9° aed 
0125% 
ferrocyanide 
i TITL + IIR = 1°8 c.c. (30 m. e + ferric chloride 
ellow coloration paly. 
— psec 448 c.c. Ms . 4 
E 40-10-10 1. 79 e.c. ee te: 
i; piped is 83 c.c. 
At 10°12 I substituted in both r.a.v. bottles a 0-025% solution (in 
0°6% saline) of KyFe (CN), and sevseavted perfusion at 10-21. 
0°025% I put 4th pair tubes from 10°25-10°55 
ferrocyanide 
i IVL+IVR =0°8 c.c. (30 m.) + ee chloride=yel- 
w coloration only. 
aries —— 424 ¢.c.) __ 53:1 
| 10251035 Keay ro M1 00. | : | 
a.v. <C 
83 flows. 
At 10°57 I substituted in both r.a.v. bottles a 0: wig egeor of 
Ky,Fe (CN), (in iis salin), =, each 2000 c.c. of w had been 
adde as 40 c.c. human smotic pressure of ren solution 
was aveore inwmcniasly te yt “ precedin 
The r.a.v. perfusion was re-started at 11-11, 
005% I put 5th pair tubes a 11°20-11°50. 
ferrocyanide 
+ urine VL+VR = 0°9 c.c. (30 m. ) + ferric eS = yellow 
via f.a.v. with a slight green tinge. 
ripe Ea ig ES eee ae 5-0: 1 
E *20-11°50 l.r oe a 
r av. me 79 c.c. ; 
Atll see I ee. Ty most of the aortic bottle esha pan id 
(the tubing was not clamped) and substituted an identical solu- 
tion save that it ‘contained 0°00625% K, Fe (CN); re aortic 
11-58, 
bottle was refilled by 
0°00625% I put 6th pair tubes from 12-5-12-20. 
ferrocyanide 
in aorta Meg VIR = 0°4 c.c. (15 m ) + ferric chloride = 
solution a deeper blue than that obtained by adding the 
ferric elas to the 0° 00625 % perfusion fluid itself. 
us if we indicate oe colorations given when the stock solution of 
ferric chloride is added, volume seb — me, respectively to the 0°00625%, 
0°0125%, 0 025% and 0: om K,Fe(CN), perfusion fluids, by the numbers 
2; 4 and 8 (the 0:05% fluid eho sc as blue as blue ink), the 
uid+the added urine 
c flow 5 times 
great as the average of the two haa) afferent vein Sows, is it is sdapnasible 
