182 Journal of the Asiatic Society of Bengal. [N.S., XVITI, 
e e he aac i 
un wire g "Fina ally, I placed : ihe water in the dish to enable the ven- 
tral body wall to absorb this. Many frogs o ceaaad on as described lived 
for more than twenty-four hours, breathing a heart pulsation continu- 
ing until —- nd. All my experiments were carefully watched and if 
p a 
nuch more 
ifficult to sliuteot these operations from the ventral side of the frog, and 
I may add that had certain previous mortiantors performed their éx- 
Aes from the dorsal side ins = d of from the ventral, there would 
ve been no dispute as to whether they va or had not failed to ligature 
ail the renal arteries 8 supplying the kidneys, since by the dorsal metho 
all are eon d plainly visible when the aorta is raised and they are put 
on the tch. 
APPENDIX G. 
Experiments on the Frog ——_ the —— that (the Venous Supply 
of the Kidneys being eliminated) I e of Pressure in the Aorta 
(4.e. Greater Pressure and aches a pe flow) yields Urine weak- 
er in Nitrogen, stronger in Chloride and greater in gy Sao tity, w — 
Post j 
a 8. )y 
stronger in loride but less in Qua : tn other words, the 
pe of the —— depends on the se the Quantity on the 
Increase of Pressure in the Aorta (Exp. Ser. A.I.P.). 
EXPERIMENT 1 — —— strengths of ur urine). Perfusion fluid 
sop ay 1 gm.! urea crystals dissolved in 2000 c.c. 06% 
e (tap. —— perfused through a large-bore cannula (out- 
20 sera ies Ist tubes from 9°15-10°45. 
IL + IR = 15 c.c. (90 m.) = Ist 20 ems 
: te of flow =178 Be. CG; rea per 30 minutes. | 
At 10: perfusion bottle raised to 30 ¢ 
30 cms. Put 2nd pair —— from 11-0-11 30. 
IL + IIR 30 m. 
ITL + IItR = 1-25 ce. (30 m.) = #40 cms. urin 
[11-45-12-15 renal artery rate of es = 115 c.c. per 30 minutes. } 
It is evident that 30 ems. gave the ptiniite flow for meré 
excretion in this experiment. Though the flow so sd = 
su 
ceed 
: Same, as will be seen, poe S decidedly 
weaker in nitrogen. This result ——- not occur if the excre- 
tion were a mere glomerular filtra 

! Experiments in which stronger soluti t ; eatdet 
not give gach 6 clear results. eis utions than this were tv 


FU eal Sige ee ee ea ect OL ae aes 


