134 1.8.C. Proceedings of the Ninth [N.S., XVII, 
= pg a series of observations were undertaken to wis the 
ious phases of reversion from the flagellate to the resistant t forms and 
ae following pri were recorded oak ne nen week from surface peice, 
on N.N.N. medium which wast found to be the most suitable fur the pur- 
ose 
" normous division and sub- division sa ats flagellates of various 
sizes and shapes by simple fission. (First two weeks. 
ne Bs so and loss of mobility ae ie “flagellate es accom ed 
by a shortening of the flagellum anda ultaneous migration of the 
blepharoplas towards the nucleus and Pt of the proce sm. 
(Third week.) 
3. jecteea and fattening phase of the parasite with the complete 
disappearance of the rudiments of the flagellum and _ closer apposition 0 of 
the ble pharoplast . i nucleus and increased vacuolation leading to the 
complete formatio a thin capsule which is cast off or aba bed. 
week. 
. Rounding off of the parasite before assuming the form found in 
the ag Saprw fours. week. 
om above clear that in the search of a transmitter the 
endless ‘arity of morphologically different flagellates, flagellates and 0 
— ould lead to — ffic _ es in identifying the parasites 
2 inculture s and therefore as e more so in are nceptete ea of 
inesite a aod = rally with atest kinds of herpat ; and in 
tiie absence of a ingle experiment prov ing the nfastivity ers these in 
susceptible ete it is a. te to Beit ; and condemn any particular 
insect a bug, a flea a phlebotomus, simply on the evidence 0 of 
Ae yet in aie “met ine e even aaveral weeks after an infective 
Note on the Cultivation of Leishmania donovani from the 
peripheral blood of persons suffering from Kala-azar.— 
By Lr.-Con. J. W. Cornwatt, I.M.S., and H. M. Lar- 
RENAIS. 
The authors related ten successive successful cultures of flagellates 
from af peripheral blood in kala-azar for purposes diagnosis and 
r 
flasks. Suggested that it be eee itely pe ded es e who have ac 
cess to numerous 2 patients with kala-azar tS ce Taeehod is really 
of value in aiding early aienad in doubtful cases 
The diagnosis of Kala-azar an pee blood culture.— 
By Brras Mouan Das 
etween March 3rd and August ai the gota nab blood of 35 cases 
of kala-azar was py claimeny > culture to N.N.N. medium. Nineteen © 
these patients had had no antimony ebaalanatis whit in the remaining 
sixteen varying amo eye of sntinine had been giv 
All those cases except two were culturally aye The average 
time when flagellates first appeared in the cultures was just under eight 
days. (Shortest period 3 days longest 22 days.) The two negative cases 
iad each undergone a com i .c. of 1% 80 
timony tartrate) but had not shown m improvement in t elin- 
ical conditio gh the peripheral blood culture in these 
negative st cases leishmania we microscopically i 
spleen smears. © cases which were positive both by per ripheral we 
e bo 
culture sett negative by microscopical examination of 
lood and spleen juic . ¥ . 
