146 18.C. Proceedings of the Ninth [N.S., XVIII, 
ciable effectand cholera evtbreaks in June to September were shen 
brief and not very intense. Those districts lying round Madras City a 
those in the south of the Presidency adjoining the Bay of tak 2 and 
the Arabian sea all presented the typical curve wich a high 
December and January, falling to a small minimum throughout ‘the na 
ar. 
The south-western interior raplpipes were represented curves 
howing a double rise—the curve being a combination of the ee already 
ri 
almost entirely unaffected “ee si south-west monsoon. Cholera begins 
to increase shortly after the vigil monsoon bursts and = eurve 
of the graphs rises abruptly to its maximum in December and January. 
The curve of South e anara Distriet which, on the above distribution, 
ought to correspond to the first Rowe was found to be v very similar to 
that of the third group ae although s as to the cause 
for this disparity of type, n o definite conclusion was criived at and the 

Further investigations were proposed on a possible relationship 
between humidity and the incidence of cholera on the lines indicated in 
Col. Gill | on the influence of humidity and temperature on the inci- 
dence of ma 
_A plea for the extended use of the Voges Proskaver 
Pest —By Lr.-Cou. Guen Liston, I.M.8. and SB. N. 
GE 

f organisms of 
the “aypho id colon group, Indole and Voges Piaskiuiet reactions stand 
pre-eminent. It is therefore aenaergee that the techniques of such routine 
reuctualae should be PPA: r and reliable. Such a technique aa 
already been described for I forsctny viz. the ‘‘ Cotton: deca ag test ”’ 
none of the methods so far recommended for Sewers ng the Vo oges 
Proskauer reaction fulfills the Sanaa ns of a routine test 
2. Accordingly we have developed a technique which ‘conforms to the 
Seecthacecns of a routine test. Gar rocedure is as follows:—The cul- 
a : 
t of 
and at the end of _ eet -four hours period of incubation @ loopfu 
of the culture is removed on white porcelain slab and mixed a loopful 
n_ th 
further lie n is require 
. In the glucose sehen the pink colour of the V.P. reaction a 
its nia scievea in “ nee o fifteen minutes but fades away almost comr 
pletely in about We have shown that if milk be need 
instead of re ned ig Saeuaiis medium, not only a ‘brighter — 
colouration is obtained but the mixture of the culture and soda solution 
solidifies and forms a dry pink spot on the slab which retains the inten 
I tai 
r more than six x 
ebserved that when tet is used v9 place of glucose in the culture 
media. a positive V.P. r n appears after a shorter period of incu uba- 
tion, and the pink rites is -hedithites oa in glucose media. 
The eee ay. of the Mills- ete 8 Bapep tre to 
Indian conditions.—By T. N. 8S. Racu os . 
The a ee of the eakasi eas phenomenon to t vital statis 
ties of any town with a ected water supply ae upon four 
essential Soeticna 
