4 UNIVERSITY OF MISSOURI STUDIES 



preparation. It was first employed, by F. Blum,^ in micro- 

 scopic technique and afterwards by Gerota in topographic 

 anatomy. Gerota^ suggested the injection of a five per 

 cent solution of formalin into the arteries and the sectioning 

 of the frozen body in the usual way. Jackson^ has recently 

 and independently shown that by the use of a fifty per cent 

 solution of formalin it is not necessary to freeze the bod)' 

 before sectioning. The advantages of this method are that 

 the organs, hardened in the exact form and position they 

 were in at the time the body was injected, do not change 

 after the sections are made ; all parts except the bones can 

 be cut with a knife, thus giving smooth, even surfaces. It 

 is also possible to decalcify after hardening with formalin, 

 but this is seldom necessary or desirable. 



6 Blum, F., Das formaldehyd als hartungsmittel. Vorlaufige 

 mitteilung. Zeitschrift f. wiss. mikroskopie, Bd. lO. 1893. 



' Gerota, D., Ueber die anwendung des formols in der topographis- 

 chen anatomie. Anat. anzeiger, Bd. 11. 1895. 



8 Jackson, C. M., A method of teaching relational anatomy. 

 Journal of the ameriean medical association, 1901. 



